Ha Tae Woong, Oh Bermseok, Kang Ji-One
Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University.
Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University;
J Vis Exp. 2020 Jun 20(160). doi: 10.3791/61583.
The electrocardiogram is a valuable tool for evaluating the cardiac conduction system. Animal research has helped generate novel genetic and pharmacological information regarding the electrocardiogram. However, making electrocardiogram measurements in small animals in vivo, such as mice, has been challenging. To this end, we used an electrocardiogram recording method in anesthetized mice with many advantages: it is a technically simple procedure, is inexpensive, has short measuring time, and is affordable, even in young mice. Despite the limitations with using anesthesia, comparisons between control and experimental groups can be performed with enhanced sensitivity. We treated mice with agonists and antagonists of the autonomic nervous system to determine the validity of this protocol and compared our results with previous reports. Our ECG protocol detected increased heart rates and QTc intervals on treatment with atropine, decreased heart rates and QTc intervals after carbachol treatment, and higher heart rates and QTc intervals with isoprenaline but did not note any change in ECG parameters on administration of propranolol. These results are supported by previous reports, confirming the reliability of this ECG protocol. Thus, this method can be used as a screening approach to making ECG measurements that otherwise would not be attempted due to high cost and technical difficulties.
心电图是评估心脏传导系统的一项重要工具。动物研究有助于产生有关心电图的新的遗传学和药理学信息。然而，在小鼠等小动物体内进行心电图测量一直具有挑战性。为此，我们在麻醉小鼠中使用了一种心电图记录方法，该方法具有诸多优点：技术操作简单、成本低廉、测量时间短，即使对于幼鼠来说也负担得起。尽管使用麻醉存在局限性，但可以以更高的灵敏度对对照组和实验组进行比较。我们用自主神经系统的激动剂和拮抗剂处理小鼠，以确定该方案的有效性，并将我们的结果与先前的报告进行比较。我们的心电图方案检测到，用阿托品治疗后心率和QTc间期增加，用卡巴胆碱治疗后心率和QTc间期降低，用异丙肾上腺素治疗后心率和QTc间期升高，但在给予普萘洛尔后未发现心电图参数有任何变化。这些结果得到了先前报告的支持，证实了该心电图方案的可靠性。因此，这种方法可作为一种筛选方法，用于进行因成本高和技术困难而原本不会尝试的心电图测量。
