Emery D L, MacHugh N D, Morrison W I
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Parasite Immunol. 1988 Jul;10(4):379-91. doi: 10.1111/j.1365-3024.1988.tb00228.x.
During the course of a lethal infection with Theileria parva (Muguga), the surface phenotypes of efferent lymphatic lymphocytes (ELL) were analysed to determine whether the parasite preferentially infected any particular subpopulation of cells. In the second week of infection, when the proportion of lymphoblasts and parasitized cells increased to 50% of the total ELL, greater than 99% of infected cells expressed T-lymphocyte markers including both BoT4 and BoT8. From day 10, a population of T-lymphocytes coexpressing BoT4 and BoT8 appeared in ELL, reaching 33% by day 14. Similar changes were observed in peripheral blood mononuclear cells (PBM) and lymph node cells (LNC). Analysis of ELL sorted into populations differing on the basis of expression of BoT4 and BoT8, revealed a higher level of parasitosis in the BoT4+ and BoT8+ lymphocytes than in the BoT4+ BoT8- or BoT4- BoT8+ populations. For comparison, the phenotypes of 28 cloned cell lines, obtained by infection of PBM with sporozoites in vitro, were examined. All of these clones exhibited T-cell markers. Nine of the clones expressed both BoT4 and BoT8; within each of these lines, BoT4 was expressed on all cells, whereas BoT8 was expressed at variable concentrations on 20-70% of cells. That BoT4+ cells were induced by T.parva (Muguga) to coexpress BoT8 was demonstrated directly by the finding that a BoT4+ BoT8- T-cell clone expressed BoT8 following infection with the parasite.
在感染致命的微小泰勒虫（穆古加株）的过程中，对传出淋巴管淋巴细胞（ELL）的表面表型进行了分析，以确定该寄生虫是否优先感染任何特定的细胞亚群。在感染的第二周，当淋巴母细胞和被寄生细胞的比例增加到ELL总数的50%时，超过99%的感染细胞表达T淋巴细胞标志物，包括BoT4和BoT8。从第10天开始，ELL中出现了共表达BoT4和BoT8的T淋巴细胞群体，到第14天达到33%。在外周血单核细胞（PBM）和淋巴结细胞（LNC）中也观察到了类似的变化。对根据BoT4和BoT8表达情况分类的ELL进行分析发现，BoT4+和BoT8+淋巴细胞中的寄生虫感染水平高于BoT4+BoT8-或BoT4-BoT8+群体。作为比较，检测了通过体外将子孢子感染PBM获得的28个克隆细胞系的表型。所有这些克隆都表现出T细胞标志物。其中9个克隆同时表达BoT4和BoT8；在每个这样的细胞系中，所有细胞都表达BoT4，而BoT8在2个细胞系中，BoT4+细胞在感染寄生虫后表达BoT8，这一发现直接证明了微小泰勒虫（穆古加株）可诱导BoT4+细胞共表达BoT8。 0%-70%的细胞中以可变浓度表达。通过发现一个BoT4+BoT8-T细胞克隆在感染寄生虫后表达BoT8，直接证明了BoT4+细胞被微小泰勒虫（穆古加株）诱导共表达BoT8。
