Czernielewski J M, Masouye I, Pisani A, Ferracin J, Auvolat D, Ortonne J P
Centre International de Recherches Dermatologiques, Sophia Antipolis, Valbonne, France.
Photodermatol. 1988 Jun;5(3):116-20.
This study evaluated the Langerhans cell density in chronically sun-exposed skin (hand) and non-exposed skin (buttock) in subjects that were divided into 4 age groups (20-40; 41-60; 61-80; greater than 81 years). Two markers (OKT-6 and anti-HLA-DR) were used to identify the Langerhans cells (LC), and their count was performed either on epidermal sheets (52 individuals) or skin sections (43 individuals). Three major findings result from this study: 1) there are more LC in the non-exposed skin than in the chronically sun-exposed area; 2) there were no age-related changes in LC counts, and 3) LC co-express the T6 and HLA-DR antigens.
本研究评估了分为4个年龄组（20 - 40岁；41 - 60岁；61 - 80岁；81岁以上）的受试者长期暴露于阳光下的皮肤（手部）和未暴露皮肤（臀部）中的朗格汉斯细胞密度。使用两种标志物（OKT - 6和抗HLA - DR）来识别朗格汉斯细胞（LC），并在表皮片（52例个体）或皮肤切片（43例个体）上进行细胞计数。本研究有三项主要发现：1）未暴露皮肤中的LC比长期暴露于阳光下的区域更多；2）LC计数没有与年龄相关的变化；3）LC共表达T6和HLA - DR抗原。
