DVGW-Technologiezentrum Wasser (TZW), Karlsruher Straße 84, D-76139 Karlsruhe, Germany.
DVGW-Technologiezentrum Wasser (TZW), Karlsruher Straße 84, D-76139 Karlsruhe, Germany.
Sci Total Environ. 2020 Nov 10;742:140529. doi: 10.1016/j.scitotenv.2020.140529. Epub 2020 Jun 25.
The emergence of antimicrobial resistances causes serious public health concerns worldwide. In recent years, the aquatic ecosystem has been recognized as a reservoir for antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). The prevalence of 11 ARGs, active against six antibiotic classes (β-lactams, aminoglycosides, tetracycline, macrolides, trimethoprim, and sulfonamides), was evaluated at a karst spring (Gallusquelle) in Germany, using molecular biological methods. In addition, fecal indicator bacteria (FIB), turbidity, electrical conductivity, spring discharge, and microbial source tracking markers specific for human, horse, chicken, and cow were determined. The ARGs most frequently detected were ermB (42.1%), tet(C) (40.8%), sul2 (39.5%), and sul1 (36.8%), which code for resistance to macrolides, tetracycline and sulfonamides, respectively. After a heavy rain event, the increase in FIB in the spring water was associated with the increase in ARGs and human-specific microbial source tracking (MST) markers. The determined correlations of the microbiological parameters, the observed overflow of a combined sewer overflow basin a few days before the increase of these parameters, and the findings of previous studies indicate that the overflow of this undersized basin located 9 km away from the spring could be a factor affecting the water quality of the karst spring. Our results provide a scientific basis for minimization of the input of fecal pollution and thus ARGs into the karst spring.
抗微生物耐药性的出现引起了全世界严重的公共卫生关注。近年来，水生生态系统已被认为是抗生素耐药细菌和抗生素耐药基因（ARGs）的储存库。使用分子生物学方法，在德国的一处喀斯特泉（Gallusquelle）评估了针对六种抗生素类别（β-内酰胺类、氨基糖苷类、四环素类、大环内酯类、甲氧苄啶和磺胺类）的 11 种 ARG 的流行情况。此外，还测定了粪便指示菌（FIB）、浊度、电导率、泉水流量和针对人、马、鸡和牛的微生物源追踪标记物。最常检测到的 ARGs 是 ermB（42.1%）、tet(C)（40.8%）、sul2（39.5%）和 sul1（36.8%），它们分别编码对大环内酯类、四环素类和磺胺类的耐药性。在一场大雨事件后，泉水中 FIB 的增加与 ARGs 和人特异性微生物源追踪（MST）标记物的增加有关。确定的微生物参数相关性、这些参数增加前几天联合污水溢流盆地的溢流以及先前研究的结果表明，位于离泉水 9 公里远的这个小型盆地的溢流可能是影响喀斯特泉水质的一个因素。我们的结果为最大限度减少粪便污染和 ARGs 进入喀斯特泉提供了科学依据。
