State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin 300050, People's Republic of China.
School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.
Bioorg Chem. 2020 Aug;101:104004. doi: 10.1016/j.bioorg.2020.104004. Epub 2020 Jun 10.
Bioactive natural products play an important role in the research and development of new drugs. In our search for bioactive natural substances as potential lead compounds for inflammation, four new (1-4) and six known (6-10) triterpenoids were acquired from Lantana camara. Using NMR and MS techniques and electronic circular dichroism (ECD) calculations, these isolates were characterized and the new compounds (1-4) were found to be euphane-type triterpenoids. The in vitro anti-inflammatory effects of all the isolates were evaluated and the more bioactive compounds were selected for the investigation of preliminary mechanism using molecular docking and Western blotting experiments, as well as the in vivo anti-inflammatory evaluation using a zebrafish model.
生物活性天然产物在新药研发中发挥着重要作用。在寻找具有抗炎活性的天然物质作为潜在先导化合物的过程中，我们从马缨丹中分离得到了四个新的（1-4）和六个已知的（6-10）三萜类化合物。通过 NMR 和 MS 技术以及电子圆二色性（ECD）计算，对这些分离物进行了表征，发现新化合物（1-4）为 euphane 型三萜类化合物。评估了所有分离物的体外抗炎活性，选择更具生物活性的化合物进行分子对接和 Western blot 实验的初步机制研究，以及利用斑马鱼模型进行体内抗炎评价。
