Lam T Y, Pickering G, Geltosky J, Siu C H
Banting and Best Department of Medical Research, C. H. Best Institute, University of Toronto, Toronto, Ontario M5G 1L6.
The Research Institute of Scripps Clinic, La Jolla, California 92037, USA.
Differentiation. 1981 Dec;20(1-3):22-28. doi: 10.1111/j.1432-0436.1981.tb01151.x. Epub 2011 Jan 21.
Pseudoplasmodia of Dictyostelium discoideum at the culmination stage were separated into two cell populations by sedimentation in a discontinuous renografin gradient. The two lighter fractions (I and II) had enzymatic activities characteristic of the anterior prestalk cells, while the heaviest fraction (III) showed enzyme activities characteristic of the posterior prespore cells. Cell-cell adhesion among prespore cells is much more resistant to EDTA dissociation than 10-h cells and prestalk cells. Fab fragments prepared from antibodies directed against a specific cell surface glycoprotein gp150 were more effective in dissociating prespore cells than prestalk cells. In addition, prespore cells contained an approximately 2-fold higher concentration of the endogenous carbohydrate binding protein discoidin-I than prestalk cells. These differences may account for the differential cohesiveness of these two cell populations and provide a basis for cell recognition and cell sorting at the slug stage.
在聚集阶段，将盘基网柄菌的伪原质团通过在不连续泛影葡胺梯度中沉降分离为两个细胞群体。两个较轻的组分（I和II）具有前柄细胞的酶活性特征，而最重的组分（III）显示出后孢子细胞的酶活性特征。孢子前体细胞之间的细胞间黏附比10小时的细胞和柄细胞对EDTA解离更具抗性。由针对特定细胞表面糖蛋白gp150的抗体制备的Fab片段在解离孢子前体细胞方面比柄细胞更有效。此外，孢子前体细胞中内源性碳水化合物结合蛋白盘基网柄菌素-I的浓度比柄细胞高约2倍。这些差异可能解释了这两个细胞群体的不同黏附性，并为蛞蝓阶段的细胞识别和细胞分选提供了基础。
