Cao Yu, Wang Jie, Germinara Giacinto Salvatore, Wang Lijuan, Yang Hong, Gao Yulin, Li Can
Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, Department of Biology and Engineering of Environment, Guiyang University, Guiyang 550005, China.
Department of the Sciences of Agriculture, Food and Environment, University of Foggia, 71122 Foggia, Italy.
Insects. 2020 Jul 1;11(7):408. doi: 10.3390/insects11070408.
is a common thrips pest of various plant flowers with host preference. Plant volatiles provide important information for host-searching in insects. We examined the behavioral responses of adults to the floral volatiles of Ellis, Bolus, Pallas, and Jacq. in a Y-tube olfactometer. adults showed significantly different preferences to these four-flower plants, with the ranking of > > ≥ . Further, 29 components were identified in the volatile profiles of , and (Z)-3-hexenyl tiglate (14.38 %), linalool (27.45 %), and (E3,E7)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (24.67 %) were the most abundant. Six-arm olfactometer bioassays showed that had significant positive responses to (Z)-3-hexenyl tiglate, linalool, and (E3,E7)-4,8,12-trimethyltrideca-1,3,7,11-tetraene tested at various concentrations, with the most attractive ones being 10 μL/μL, 10 μL/μL and 100 μg/μL for each compound, respectively. In pairing of these three compounds at their optimal concentrations, showed the preference ranking of (Z)-3-hexenyl tiglate > linalool > (E3,E7)-4,8,12-trimethyltrideca-1,3,7,11-tetraene. Large numbers of have been observed on flowers in the field, which might be caused by the high attraction of this pest to floral volatiles shown in the present study. Our findings shed light on the olfactory cues routing host plant searching behavior in , providing important information on how targets particular host plants. The high attractiveness of the main compounds (e.g., linalool, (E3,E7)-4,8,12-trimethyltrideca-1,3,7,11-tetraene, particular (Z)-3-hexenyl tiglate) identified from volatiles of flowers may be exploited further to develop novel monitoring and control tools (e.g., lure and kill strategies) against this flower-inhabiting thrips pest.
是一种对多种植物花朵有寄主偏好的常见蓟马害虫。植物挥发物为昆虫寻找寄主提供重要信息。我们在Y型嗅觉仪中检测了成虫对埃利斯花、博卢斯花、帕拉斯花和雅克花的花香挥发物的行为反应。成虫对这四种花卉植物表现出显著不同的偏好，偏好顺序为 > > ≥ 。此外，在、和的挥发物成分中鉴定出29种成分，其中(Z)-3-己烯基惕各酸酯(14.38%)、芳樟醇(27.45%)和(E3,E7)-4,8,12-三甲基十三碳-1,3,7,11-四烯(24.67%)含量最高。六臂嗅觉仪生物测定表明，成虫对不同浓度的(Z)-3-己烯基惕各酸酯、芳樟醇和(E3,E7)-4,8,12-三甲基十三碳-1,3,7,11-四烯有显著的正向反应，每种化合物最具吸引力的浓度分别为10 μL/μL、10 μL/μL和100 μg/μL。当这三种化合物以最佳浓度配对时，成虫表现出的偏好顺序为(Z)-3-己烯基惕各酸酯 > 芳樟醇 > (E3,E7)-4,8,12-三甲基十三碳-1,3,7,11-四烯。在田间观察到大量成虫出现在花上，这可能是由于本研究中该害虫对花的挥发物具有高度吸引力所致。我们的研究结果揭示了引导成虫寻找寄主植物行为的嗅觉线索，为了解成虫如何定位特定寄主植物提供了重要信息。从花的挥发物中鉴定出的主要化合物（如芳樟醇、(E3,E7)-4,8,12-三甲基十三碳-1,3,7,11-四烯，特别是(Z)-3-己烯基惕各酸酯）的高吸引力，可能会被进一步利用来开发针对这种栖息在花上的蓟马害虫的新型监测和控制工具（如诱捕和杀灭策略）。
