Zhang Tao, Yang Yuping, Maggi Filippo, Jiang Feiyu, Yuan Rongrong, Huang Lujie, Zhang Xueyan, Cao Yu, Gao Yulin
School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guiyang University, Guiyang 550005, China.
Insects. 2025 Jun 16;16(6):633. doi: 10.3390/insects16060633.
Volatile cues are important in the host detection and discrimination of phytophagous insects, allowing them to find suitable hosts. Here, the electroantennography (EAG) and behavioral responses of female to the floral volatiles of different plants, L. (Magnoliales: Magnoliaceae), Bolus (Asterales: Asteraceae), Baker (Liliales: Liliaceae), and Thunb. (Rosales: Rosaceae), were studied. Y-tube olfactometer bioassays revealed that was the preferred host for . Fifty-two compounds were identified from the volatile profile of by gas chromatography-mass spectrometry analysis, of which -elemen (15.39%), bicyclogermacren (11.99%), and ()--bisabolene (6.05%) showed the highest relative contents. The EAG bioassays revealed that the antennae of could perceive these tested volatile compounds at different concentrations. In six-arm olfactometer bioassays, showed significant positive responses to -elemen and ()--bisabolene at various concentrations, and 10 μg/μL was found to be the most attractive concentration for -elemen, and 50 and 100 μg/μL for ()--bisabolene. Based on the four-arm olfactometer bioassays, -elemen was more attractive than ()--bisabolene to when compared at their optimal concentrations. Therefore, could perceive and distinguish the floral volatiles from the preferred host plant (). These findings assist in better understanding the mechanism of host preferences of from a chemoecological perspective. In particular, -elemen showed the greatest potential to be developed as a novel attractant for the monitoring and control of .
挥发性线索在植食性昆虫的寄主检测和辨别中很重要，能使它们找到合适的寄主。在此，研究了雌性[昆虫名称]对不同植物的花香挥发物的触角电位（EAG）和行为反应，这些植物分别是木兰科木兰目[植物1名称]、菊科菊目[植物2名称]、百合科百合目[植物3名称]和蔷薇科蔷薇目[植物4名称]。Y型嗅觉仪生物测定表明，[植物名称]是[昆虫名称]的首选寄主。通过气相色谱 - 质谱分析从[植物名称]的挥发物谱中鉴定出52种化合物，其中β-榄香烯（15.39%）、双环吉马烯（11.99%）和（E）-β-石竹烯（6.05%）的相对含量最高。EAG生物测定表明，[昆虫名称]的触角能感知不同浓度的这些受试挥发性化合物。在六臂嗅觉仪生物测定中，[昆虫名称]对不同浓度的β-榄香烯和（E）-β-石竹烯表现出显著的正向反应，发现10μg/μL对β-榄香烯最具吸引力，50和100μg/μL对（E）-β-石竹烯最具吸引力。基于四臂嗅觉仪生物测定，在最佳浓度下比较时，β-榄香烯对[昆虫名称]比（E）-β-石竹烯更具吸引力。因此，[昆虫名称]能够感知并区分来自首选寄主植物（[植物名称]）的花香挥发物。这些发现有助于从化学生态学角度更好地理解[昆虫名称]寄主偏好的机制。特别是，β-榄香烯作为一种用于监测和控制[昆虫名称]的新型引诱剂具有最大的开发潜力。
