Sánchez-Rodríguez Carolina, Peiró Concepción, Rodríguez-Mañas Leocadio, Nevado Julián
Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, Villaviciosa de Odón, 28670 Madrid, Spain.
Department of Pharmacology, School of Medicine, Instituto de Investigaciones Sanitarias IdiPAZ, Universidad Autónoma de Madrid, 28029 Madrid, Spain.
Antioxidants (Basel). 2020 Jul 1;9(7):572. doi: 10.3390/antiox9070572.
We investigated the cytoprotective role of the dietary polyphenols on putative damage induced by Amadori adducts in Human Peritoneal Mesothelial Cells (HPMCs). Increased accumulation of early products of non-enzymatic protein glycation-Amadori adducts-in the peritoneal dialysis fluid due to their high glucose, induces severe damage in mesothelial cells during peritoneal dialysis. Dietary polyphenols reportedly have numerous health benefits in various diseases and have been used as an efficient antioxidant in the context of several oxidative stress-related pathologies. HPMCs isolated from different patients were exposed to Amadori adducts (highly glycated haemoglobin, at physiological concentrations), and subsequently treated with several polyphenols, mostly presented in our Mediterranean diet. We studied several Amadori-induced effects in pro-apoptotic and oxidative stress markers, as well as the expression of several pro-inflammatory genes (nuclear factor-kappaB, NF-kB; inducible Nitric Oxide synthetase, iNOS), different caspase-activities, level of P53 protein or production of different reactive oxygen species in the presence of different polyphenols. In fact, cytoprotective agents such as dietary polyphenols may represent an alternate approach to protect mesothelial cells from the cytotoxicity of Amadori adducts. The interference with the Amadori adducts-triggered mechanisms could represent a therapeutic tool to reduce complications associated with peritoneal dialysis in the peritoneum, helping to maintain peritoneal membrane function longer in patients undergoing peritoneal dialysis.
我们研究了膳食多酚对人腹膜间皮细胞（HPMCs）中由阿马多里加合物诱导的假定损伤的细胞保护作用。由于腹膜透析液中高糖导致非酶蛋白糖基化早期产物——阿马多里加合物的积累增加，在腹膜透析过程中会对间皮细胞造成严重损伤。据报道，膳食多酚在各种疾病中具有多种健康益处，并且在几种与氧化应激相关的病理情况下已被用作有效的抗氧化剂。从不同患者分离的HPMCs暴露于阿马多里加合物（生理浓度的高度糖化血红蛋白），随后用几种多酚进行处理，这些多酚大多存在于我们的地中海饮食中。我们研究了几种阿马多里诱导的对促凋亡和氧化应激标志物的影响，以及几种促炎基因（核因子-κB，NF-κB；诱导型一氧化氮合酶，iNOS）的表达、不同的半胱天冬酶活性、P53蛋白水平或在不同多酚存在下不同活性氧的产生。事实上，膳食多酚等细胞保护剂可能代表一种保护间皮细胞免受阿马多里加合物细胞毒性影响的替代方法。干扰阿马多里加合物触发的机制可能代表一种治疗工具，以减少与腹膜透析相关的腹膜并发症，有助于在接受腹膜透析的患者中更长时间地维持腹膜功能。
