Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Am Klinikum 1, D-07747 Jena, Germany.
Croatian Institute for Brain Research, School of Medicine University of Zagreb, Salata 12, 10000 Zagreb, Croatia.
Int J Mol Sci. 2020 Jul 1;21(13):4716. doi: 10.3390/ijms21134716.
To test and introduce effective and less toxic breast cancer (BC) treatment strategies, animal models, including murine BC cell lines, are considered as perfect platforms. Strikingly, the knowledge on the genetic background of applied BC cell lines is often sparse though urgently necessary for their targeted and really justified application.
In this study, we performed the first molecular cytogenetic characterization for three murine BC cell lines C-127I, EMT6/P and TA3 Hauschka. Besides fluorescence in situ hybridization-banding, array comparative genomic hybridization was also applied. Thus, overall, an in silico translation for the detected imbalances and chromosomal break events in the murine cell lines to the corresponding homologous imbalances in humans could be provided. The latter enabled a comparison of the murine cell line with human BC cytogenomics.
All three BC cell lines showed a rearranged karyotype at different stages of complexity, which can be interpreted carefully as reflectance of more or less advanced tumor stages.
Accordingly, the C-127I cell line would represent the late stage BC while the cell lines EMT6/P and TA3 Hauschka would be models for the premalignant or early BC stage and an early or benign BC, respectively. With this cytogenomic information provided, these cell lines now can be applied really adequately in future research studies.
为了测试和引入有效且毒性较小的乳腺癌（BC）治疗策略，动物模型，包括鼠类 BC 细胞系，被认为是完美的平台。然而，尽管对于这些细胞系的靶向和合理应用非常必要，但有关应用的 BC 细胞系遗传背景的知识往往非常匮乏。
在这项研究中，我们对三种鼠类 BC 细胞系 C-127I、EMT6/P 和 TA3 Hauschka 进行了首次分子细胞遗传学特征分析。除了荧光原位杂交带型分析外，还应用了比较基因组杂交阵列。因此，可以对在鼠类细胞系中检测到的不平衡和染色体断裂事件进行计算机翻译，将其转换为人类相应的同源不平衡。后者可以将鼠类细胞系与人类 BC 细胞遗传学进行比较。
三种 BC 细胞系均表现出不同程度复杂性的重排核型，可以仔细解释为或多或少高级肿瘤阶段的反映。
因此，C-127I 细胞系代表晚期 BC，而 EMT6/P 和 TA3 Hauschka 细胞系分别代表前恶性或早期 BC 阶段和早期或良性 BC 阶段。有了这些提供的细胞遗传学信息，这些细胞系现在可以在未来的研究中得到更充分的应用。
