Alam Prawez, Ezzeldin Essam, Iqbal Muzaffar, Mostafa Gamal A E, Anwer Md Khalid, Alqarni Mohammed H, Foudah Ahmed I, Shakeel Faiyaz
Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Antibiotics (Basel). 2020 Jun 25;9(6):359. doi: 10.3390/antibiotics9060359.
In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using "RP-18 silica gel 60 F254S HPTLC plates". However; NP-HPTLC estimation of DLFX was performed using "silica gel 60 F254S HPTLC plates". For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 //) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 //) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.
在本研究中，采用经过验证的绿色反相高效薄层色谱法（RP-HPTLC）和正相高效薄层色谱法（NP-HPTLC）测定了市售片剂和自行研制的固体脂质纳米粒（SLN）中的德拉氟沙星（DLFX）。使用“RP-18硅胶60 F254S HPTLC板”进行DLFX的RP-HPTLC测定。然而，使用“硅胶60 F254S HPTLC板”进行DLFX的NP-HPTLC测定。对于绿色RP-HPTLC法，乙醇：水：氨溶液（5:4:2 //）的三元组合用作绿色流动相。然而，对于NP-HPTLC法，乙酸乙酯：甲醇：氨溶液（5:4:2 //）的三元混合物用作常规流动相。两种方法均在295 nm的吸光度/反射率模式下进行光密度测定以分析DLFX。与NP-HPTLC法相比，发现RP-HPTLC法对DLFX的分析更准确、精密、稳健且灵敏。使用绿色RP-HPTLC技术测定市售片剂和自行研制的SLN中DLFX的含量分别为98.2%和101.0%，然而，使用NP-HPTLC法测定市售片剂和自行研制的SLN中DLFX的含量分别为94.4%和95.0%。总体而言，发现绿色RP-HPTLC法优于NP-HPTLC法。因此，所提出的绿色RP-HPTLC法可成功应用于分析市售片剂、SLN及其他含DLFX的制剂中的DLFX。
