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验证的高效薄层色谱法及抗氧化活性用于发酵茶（阿萨姆变种）中儿茶素的质量控制。

Validated HPTLC and antioxidant activities for quality control of catechin in a fermented tea ( var. assamica).

作者信息

Thammarat Phanit, Sirilun Sasithorn, Phongpradist Rungsinee, Raiwa Araya, Pandith Hataichanok, Jiaranaikulwanitch Jutamas

机构信息

Department of Pharmaceutical Sciences Faculty of Pharmacy Chiang Mai University Chiang Mai Thailand.

Innovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals Faculty of Pharmacy Chiang Mai University Chiang Mai Thailand.

出版信息

Food Sci Nutr. 2021 May 4;9(6):3228-3239. doi: 10.1002/fsn3.2285. eCollection 2021 Jun.

DOI:10.1002/fsn3.2285
PMID:34136187
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8194912/
Abstract

Miang, a Thai traditional fermented tea ( var. assamica), is exploited as nutraceutical and cosmeceutical ingredients despite limited standardization studies. Thus, this research aimed to develop a simple and rapid method for miang quality control using catechin and high-performance thin-layer chromatography (HPTLC) validated according to the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) and the Association of Official Analytical Collaboration (AOAC). The developing solvent consisting of toluene: ethyl acetate: acetone: formic acid (6:6:6:1 v/v/v/v) showed acceptable specificity with value of 0.54 ± 0.02 and linearity with correlation coefficient of 0.9951. The recovery was 98.84%-103.53%, and the RSD of intra- and inter-day precision was 0.70%-3.00% and 1.93%-4.94%, respectively. Miang ethyl acetate fraction is suggested to be attractive ingredient due to rich catechin (25.78 ± 0.53%), prolonged stability at 40 C, and strong antioxidants determined by the assays of ABTS (IC = 3.32 ± 0.74 mg/ml), FRAP (89.05 ± 15.49 mg equivalent of FeSO/g), and inhibition of lipid peroxidation (IC = 4.36 ± 0.67 mg/ml).

摘要

Miang是一种泰国传统发酵茶（阿萨姆变种），尽管标准化研究有限，但它被用作营养保健品和化妆品原料。因此，本研究旨在开发一种简单快速的方法，用于使用儿茶素和根据人用药品注册技术要求国际协调理事会（ICH）和官方分析协作协会（AOAC）验证的高效薄层色谱法（HPTLC）对Miang进行质量控制。由甲苯：乙酸乙酯：丙酮：甲酸（6:6:6:1 v/v/v/v）组成的展开溶剂显示出可接受的特异性， 值为0.54±0.02，线性相关系数为0.9951。回收率为98.84%-103.53%，日内和日间精密度的相对标准偏差分别为0.70%-3.00%和1.93%-4.94%。由于富含儿茶素（25.78±0.53%）、在40℃下稳定性延长以及通过ABTS（IC = 3.32±0.74 mg/ml）、FRAP（89.05±15.49 mg FeSO4当量/g）测定和脂质过氧化抑制（IC = 4.36±0.67 mg/ml）确定的强抗氧化剂，Miang乙酸乙酯馏分被认为是一种有吸引力的成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/a5209a6b3e8a/FSN3-9-3228-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/2feae6a82020/FSN3-9-3228-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/090878a4b6cb/FSN3-9-3228-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/9b46e1e496d4/FSN3-9-3228-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/17282f176a9a/FSN3-9-3228-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/a20706ddda43/FSN3-9-3228-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/2eed1d3e766d/FSN3-9-3228-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/97f149d9fce5/FSN3-9-3228-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/04b97f3db906/FSN3-9-3228-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/a5209a6b3e8a/FSN3-9-3228-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/2feae6a82020/FSN3-9-3228-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/090878a4b6cb/FSN3-9-3228-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/9b46e1e496d4/FSN3-9-3228-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/17282f176a9a/FSN3-9-3228-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/a20706ddda43/FSN3-9-3228-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/2eed1d3e766d/FSN3-9-3228-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/97f149d9fce5/FSN3-9-3228-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/04b97f3db906/FSN3-9-3228-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cd4/8194912/a5209a6b3e8a/FSN3-9-3228-g006.jpg

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