Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland.
Department of Immunology, Faculty of Chemistry, St. Jude Children's Research Hospital, Memphis, USA.
J Sep Sci. 2018 Dec;41(23):4304-4314. doi: 10.1002/jssc.201800506. Epub 2018 Nov 2.
The review presents general principles for choosing optimal conditions for ecdysteroid separation, identification, and isolation using HPLC/TLC techniques in RP, NP-HILIC or NP modes. Analytics of ecdyteroids pose a still insufficiently resolved problem. Plant-derived ecdysteroids are a point of interest of pharmaceutical industry and sport medicine due to their postulated adaptogenic and anabolic properties. In insects, ecdysteroids regulate larval transformation. Maral root (Rhaponticum carthamoides, Leuzea carthamoides), traditional Siberian folk-medicine plant used as stimulant to boost overall health and fitness, is a particularly rich source of a wide variety of phytoecdysteroids. The similarity of molecular structures of ecdysteroids present in its extracts together with high content of unrelated compounds of similar chromatographic characteristics makes optimization of separation, identification and isolation of ecdysteroids a difficult analytical task. In that respect, two-dimensional separations, two-dimensional separations, 2D HPLC or 2D TLC, could be of use. For identification, the hyphenated techniques are particularly important. Thus, comprehensive overview of MS spectral parameters of ecdysteroids is provided. Described principles could easily be applied for separation of ecdysteroids in extracts from other sources. They are also useful for development of separation procedures for isolation of ecysteroids in preparative-scale applications.
该综述介绍了使用 HPLC/TLC 技术在反相、正相 HILIC 或正相模式下分离、鉴定和分离蜕皮甾酮的最佳条件的一般原则。蜕皮甾酮的分析仍然是一个尚未解决的问题。植物来源的蜕皮甾酮因其被认为具有适应原和合成代谢特性而成为制药工业和运动医学的关注点。在昆虫中，蜕皮甾酮调节幼虫转化。马鹿根（Rhaponticum carthamoides，Leuzea carthamoides），一种传统的西伯利亚民间医学植物，用作刺激物来促进整体健康和健身，是各种植物蜕皮甾酮的丰富来源。其提取物中存在的蜕皮甾酮的分子结构相似性以及具有相似色谱特征的无关化合物的高含量使得蜕皮甾酮的分离、鉴定和分离的优化成为一项具有挑战性的分析任务。在这方面，二维分离、二维 HPLC 或二维 TLC 可能会有所帮助。对于鉴定，特别是重要的是联用技术。因此，提供了蜕皮甾酮的 MS 光谱参数的综合概述。所描述的原则可以很容易地应用于从其他来源的提取物中分离蜕皮甾酮。它们也可用于开发用于分离制备规模应用中合成甾酮的分离程序。
