Thrombosis and Hemostasis Research Unit, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.
Neonatal Intensive Care Unit, Department of Pediatrics and Adolescent Medicine, Aarhus University Hospital, Aarhus, Denmark.
Platelets. 2021 Jul 4;32(5):626-632. doi: 10.1080/09537104.2020.1786040. Epub 2020 Jul 6.
Platelet function in neonates is sparsely investigated. The majority of previous studies investigated platelets in umbilical cord (UC) blood rather than in peripheral blood.We included 20 term neonates and sampled UC blood and peripheral blood within 20 min and 24 h after birth. Platelet count and mean platelet volume (MPV) were measured. Platelet surface glycoproteins (GP) and platelet activation (bound fibrinogen, CD63 and -selectin) after agonist stimulation were examined by flow cytometry. Platelet aggregation was evaluated by impedance aggregometry. The significance level was set after Bonferroni correction.Platelet count and MPV did not differ between UC and peripheral blood (-values >0.08). Expression of platelet surface GP was similar in UC and peripheral blood (all -values >0.02). Platelet activation was lower in UC blood than in peripheral blood for bound fibrinogen (four out of eight -values <0.001) but did not differ for CD63 (all -values >0.01) or -selectin (all -values >0.01). Platelet aggregation was significantly higher in UC than in peripheral blood (-values <0.001).In conclusion, platelet count, MPV and expression of platelet surface GP measured in term neonatal UC blood represented that of peripheral blood. Platelet activation and aggregation in UC blood did not reflect that of peripheral blood.
新生儿的血小板功能研究较少。以前的大多数研究都调查了脐带血（UC）中的血小板，而不是外周血中的血小板。我们纳入了 20 例足月新生儿，在出生后 20 分钟和 24 小时内分别采集 UC 血和外周血。测量血小板计数和平均血小板体积（MPV）。通过流式细胞术检测血小板表面糖蛋白（GP）和血小板激活（结合纤维蛋白原、CD63 和 -选择素）。通过阻抗聚集法评估血小板聚集。在 Bonferroni 校正后设定显著性水平。UC 和外周血之间的血小板计数和 MPV 没有差异（-值>0.08）。UC 和外周血中的血小板表面 GP 表达相似（所有-值>0.02）。UC 血中的血小板激活低于外周血中的血小板激活，纤维蛋白原结合（八个-值中有四个<0.001），但 CD63（所有-值>0.01）或 -选择素（所有-值>0.01）无差异。UC 中的血小板聚集明显高于外周血（-值<0.001）。总之，在足月新生儿 UC 血中测量的血小板计数、MPV 和血小板表面 GP 的表达反映了外周血的情况。UC 血中的血小板激活和聚集并不能反映外周血的情况。
