Kollia Maria, Iacovidou Nicoletta, Iliodromiti Zoi, Pouliakis Abraham, Sokou Rozeta, Mougiou Vasiliki, Boutsikou Maria, Politou Marianna, Boutsikou Theodora, Valsami Serena
Neonatal Department, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
2nd Department of Pathology, "Attikon" University Hospital, National and Kapodistrian University of Athens, Athens, Greece.
Front Pediatr. 2022 Sep 8;10:946932. doi: 10.3389/fped.2022.946932. eCollection 2022.
Platelet function of fetal growth restricted (FGR) neonates remains a field of debate. Platelet function analyzer (PFA-100) offers a quantitative assessment of primary, platelet-related hemostasis. Our aim was to examine platelet function using PFA-100 in FGR neonates and associate our results with perinatal parameters.
PFA-100 was applied on 74 FGR neonates, 48 full-term (>37 weeks' gestation) and 26 preterm neonates (<37 weeks). The control group consisted of 118 healthy neonates. Two closure times (CTs) with COL/EPI and COL/ADP cartridges were determined on cord blood samples for each subject. Statistical analysis was performed by SAS 9.4. The statistical significance level was set at 0.05 and all tests were two-tailed.
COL/EPI CTs were prolonged in FGR (median 132 s, IQR 95-181 s) compared with control neonates (median 112.5 s, IQR 93-145 s), = 0.04. Median COL/EPI CT for term and preterm FGR neonates was 126 s (IQR 90-157 s) and 137 s (IQR 104-203), respectively ( = 0.001), and COL/ADP CT was 70 s (IQR 62-80 s) for term and 75 s (IQR 68-82 s) for preterm FGR neonates ( = 0.08). Among FGR neonates, COL/EPI CT was related with delivery time (with preterm neonates exhibiting prolonged COL/EPI CTs), = 0.05. No correlation was proved between both CTs and hematological parameters in FGR neonates.
FGR neonates showed impaired platelet function PFA-100, with preterm FGR neonates confronting the greatest risk. Prolonged COL/EPI CTs in FGR neonates seemed to be independent of hematological parameters and could warn for closer evaluation during the first days of their lives.
胎儿生长受限（FGR）新生儿的血小板功能仍是一个存在争议的领域。血小板功能分析仪（PFA - 100）可对原发性血小板相关止血进行定量评估。我们的目的是使用PFA - 100检测FGR新生儿的血小板功能，并将我们的结果与围产期参数相关联。
对74例FGR新生儿、48例足月儿（孕周>37周）和26例早产儿（孕周<37周）应用PFA - 100。对照组由118例健康新生儿组成。对每个受试者的脐血样本测定使用COL/EPI和COL/ADP检测卡的两个封闭时间（CT）。采用SAS 9.4进行统计分析。统计显著性水平设定为0.05，所有检验均为双侧检验。
与对照新生儿（中位数112.5秒，四分位数间距93 - 145秒）相比，FGR新生儿的COL/EPI CT延长（中位数132秒，四分位数间距95 - 181秒），P = 0.04。足月儿和早产儿FGR新生儿的COL/EPI CT中位数分别为126秒（四分位数间距90 - 157秒）和137秒（四分位数间距104 - 203秒）（P = 0.001），足月儿FGR新生儿的COL/ADP CT为70秒（四分位数间距62 - 80秒），早产儿FGR新生儿为75秒（四分位数间距68 - 82秒）（P = 0.08）。在FGR新生儿中，COL/EPI CT与分娩时间相关（早产儿的COL/EPI CT延长），P = 0.05。FGR新生儿的两种CT与血液学参数之间均未证实存在相关性。
FGR新生儿显示出PFA - 100检测的血小板功能受损，其中早产儿FGR新生儿面临的风险最大。FGR新生儿COL/EPI CT延长似乎与血液学参数无关，可警示在其生命最初几天进行更密切的评估。
