Lyle D B, Ayotte R D, Sheppard A R, Adey W R
Research Service, Jerry L. Pettis Memorial Veterans Hospital, Loma Linda, CA 92357.
Bioelectromagnetics. 1988;9(3):303-13. doi: 10.1002/bem.2250090311.
A significant 25% inhibition (P less than .005) of allogeneic cytotoxicity of the target cell MPC-11 by the murine cytotoxic T-lymphocyte line CTLL-1 was observed when the 4-h cytotoxicity assay was conducted immediately following a 48-h pre-exposure of the effector lymphocytes to a 10-mV/cm (rms) 60-Hz sinusoidal electric field. At 1.0 mV/cm a significant 19% inhibition (P less than .0005) was seen. At 0.1 mV/cm a nonsignificant 7% inhibition of cytotoxicity was noted. When the 4-h cytotoxicity assay was conducted in the presence of the field using previously unexposed effector lymphocytes, cytotoxicity was not significantly reduced. Cell proliferation in the presence of interleukin-2 was unaffected by the field. These data suggest a dose response and threshold (between 0.1 and 1.0 mV/cm) for inhibition of cytotoxicity in clonal T-lymphocytes by exposure to a 60-Hz sinusoidal electric field. These results suggest mechanisms by which 60-Hz electric fields could affect the function of cells of the immune system.
当效应淋巴细胞在暴露于10毫伏/厘米（均方根值）60赫兹正弦电场48小时后立即进行4小时细胞毒性试验时，观察到小鼠细胞毒性T淋巴细胞系CTLL-1对靶细胞MPC-11的同种异体细胞毒性有显著的25%抑制作用（P小于0.005）。在1.0毫伏/厘米时，观察到显著的19%抑制作用（P小于0.0005）。在0.1毫伏/厘米时，观察到细胞毒性有7%的非显著抑制作用。当使用先前未暴露的效应淋巴细胞在电场存在下进行4小时细胞毒性试验时，细胞毒性没有显著降低。在白细胞介素-2存在下的细胞增殖不受电场影响。这些数据表明，暴露于60赫兹正弦电场对克隆T淋巴细胞的细胞毒性抑制存在剂量反应和阈值（在0.1和1.0毫伏/厘米之间）。这些结果提示了60赫兹电场可能影响免疫系统细胞功能的机制。
