氯化铜对猫杯状病毒的抗病毒作用及其与利巴韦林的体外协同作用。
Antiviral effect of copper chloride on feline calicivirus and synergy with ribavirin in vitro.
机构信息
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 666 Liuying West Road, Changchun, 130122, China.
出版信息
BMC Vet Res. 2020 Jul 6;16(1):231. doi: 10.1186/s12917-020-02441-0.
BACKGROUND
Feline calicivirus (FCV) is a common and highly prevalent pathogen causing upper respiratory diseases in kittens and felines in recent years. Due to the substantial genetic variability of the viral genes, existing vaccines cannot provide complete protection. Therefore, research on FCV antiviral drugs has received much attention.
RESULTS
In this study, we found that copper chloride had dose-dependent antiviral effects on FCV in F81 cells. We also found that the combination of copper chloride and ribavirin had a synergistic protective effect against FCV in F81 cells. In contrast, the combination of copper chloride and horse anti-FCV immunoglobulin F (ab') showed an antagonistic effect, likely because copper chloride has an effect on F (ab') immunoglobulin; however, further research is needed to clarify this supposition.
CONCLUSIONS
In summary, we found that copper chloride had low cytotoxicity and significant antiviral effects on FCV in F81 cells, providing a new drug candidate for the prevention and treatment of FCV infection.
背景
猫杯状病毒（FCV）是一种常见且高度流行的病原体，近年来导致幼猫和猫科动物上呼吸道疾病。由于病毒基因的遗传变异性很大，现有的疫苗不能提供完全的保护。因此，FCV 抗病毒药物的研究受到了广泛关注。
结果
在这项研究中，我们发现氯化铜对 F81 细胞中的 FCV 具有剂量依赖性的抗病毒作用。我们还发现，氯化铜和利巴韦林联合使用对 F81 细胞中的 FCV 具有协同保护作用。相比之下，氯化铜和马抗 FCV 免疫球蛋白 F（ab'）的联合使用表现出拮抗作用，这可能是因为氯化铜对 F（ab'）免疫球蛋白有影响；然而，需要进一步的研究来阐明这一假设。
结论
综上所述，我们发现氯化铜对 F81 细胞中的 FCV 具有低细胞毒性和显著的抗病毒作用，为预防和治疗 FCV 感染提供了一种新的药物候选物。
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