Sakanaka M, Magari S, Shibasaki T, Yokokawa K
Department of Anatomy, Osaka Medical College, Japan.
Brain Res. 1988 Oct 1;471(2):175-82. doi: 10.1016/0165-3806(88)90097-1.
The first set of the present experiments was designed to investigate the postnatal development of corticotropin releasing factor-like immunoreactivity (CRFI) in the rat cerebral cortex by means of cobalt-enhanced immunohistochemistry. Results showed the occurrence of CRFI fibres before cells in the developing rat cerebral cortex with and without colchicine treatment, suggesting that some CRFI cells in subcortical regions may project to the cerebral cortex. In the second set of experiments, ipsilateral double-labelled cells which contained both retrogradely transported horseradish peroxidase (HRP) and CRFI were observed in the zona incerta, subincertal nucleus, lateral hypothalamic area, perifornical hypothalamic area, and in the dorsal hypothalamic area after unilateral HRP injections into the cerebral cortex. These findings indicate the existence of corticopetal CRFI-containing projections arising from the above areas.
本实验的第一组旨在通过钴增强免疫组织化学法研究大鼠大脑皮质促肾上腺皮质激素释放因子样免疫反应性（CRFI）的产后发育。结果显示，无论有无秋水仙碱处理，发育中的大鼠大脑皮质中CRFI纤维均先于细胞出现，这表明皮质下区域的一些CRFI细胞可能投射至大脑皮质。在第二组实验中，单侧将辣根过氧化物酶（HRP）注入大脑皮质后，在未定带、未定核下、下丘脑外侧区、下丘脑穹窿周区以及下丘脑背侧区观察到同侧双标记细胞，这些细胞同时含有逆行转运的辣根过氧化物酶（HRP）和CRFI。这些发现表明，上述区域存在向皮质投射的含CRFI的纤维。
