Sakanaka M, Shibasaki T, Lederis K
Brain Res. 1987 Jun 23;414(1):68-76. doi: 10.1016/0006-8993(87)91326-6.
Using a modified cobalt-glucose oxidase-diaminobenzidine (Co-GOD) method in a combination of horseradish peroxidase (HRP) retrograde tracing and immunohistochemistry, a widespread localization of corticotropin releasing factor-like immunoreactive (CRFI) structures in the rat inferior colliculus (IC), and a CRFI-containing pathway from the subthalamus and the hypothalamus to the IC have been observed. By means of the modified Co-GOD method, CRFI cells were detected in almost all the subdivisions of the IC, including the dorsomedial part of the central nucleus, the ventrolateral part of the central nucleus, the pericentral nucleus and the external nucleus. Neural processes with CRFI were observed in all of the above areas. Following HRP injection into the IC, double-labeled cells which contained a homogeneous brown immunoreaction product of CRF and a granular black reaction product of retrogradely transported HRP were identified in the lateral hypothalamic area (LH), zona incerta (ZI) and perifornical hypothalamic area (PeF). These double-labeled cells provide direct evidence for CRFI projections from the LH, ZI and PeF to the IC. Thus, the present study supports the view that CRF may act as a neurotransmitter or neuromodulator in the brain.
采用改良的钴 - 葡萄糖氧化酶 - 二氨基联苯胺（Co - GOD）方法，结合辣根过氧化物酶（HRP）逆行追踪和免疫组织化学技术，在大鼠下丘（IC）中观察到促肾上腺皮质激素释放因子样免疫反应性（CRFI）结构的广泛分布，以及一条从丘脑底核和下丘脑到IC的含CRFI通路。通过改良的Co - GOD方法，在IC的几乎所有亚区均检测到CRFI细胞，包括中央核的背内侧部、中央核的腹外侧部、中央周核和外侧核。在上述所有区域均观察到有CRFI的神经突起。将HRP注入IC后，在下丘脑外侧区（LH）、未定带（ZI）和下丘脑穹窿周区（PeF）中鉴定出双标记细胞，这些细胞含有CRF均匀的棕色免疫反应产物和逆行运输的HRP颗粒状黑色反应产物。这些双标记细胞为从LH、ZI和PeF到IC的CRFI投射提供了直接证据。因此，本研究支持CRF可能在脑中作为神经递质或神经调质起作用的观点。
