Kekow J, Kern P, Rautmann A, Gross W L
Department of Internal Medicine, Christian Albrecht University, Kiel, Federal Republic of Germany.
Cancer Detect Prev. 1988;12(1-6):231-6.
In AIDS elevated serum Ig levels and autoimmune phenomena indicate that B cells are also involved. The human immunodeficiency virus (HIV) can be cultivated in B cells, and HIV can stimulate B cells. In order to characterize the B-cell dysfunction and conditions for modulating it, functional studies with highly purified B cells were done in four patients with PGL and HIV-positive sera. Data were compared with those from patients with AIDS and normal controls. The experiments consisted of an in vitro study of the differentiation response (IgM/G secretion into culture supernatants) to a T cell-independent polyclonal B-cell activator (PBA) [Klebsiella pneumoniae, KlebsM]. A weak increase in IgM/G levels under stimulatory conditions was characteristic. Addition of recombinant interleukin 2 (rIL2) failed to increase the spontaneous Ig levels. However, coculture experiments using KlebsM and rIL2 resulted in Ig levels like those known from healthy individuals. Patients with frank AIDS did not respond with increased IgG secretion. This indicates that the abnormal B-cell differentiation response to PBAs can be modulated by rIL2 in patients with PGL and partly in AIDS (only IgM).
在艾滋病中，血清免疫球蛋白水平升高和自身免疫现象表明B细胞也参与其中。人类免疫缺陷病毒（HIV）可在B细胞中培养，且HIV能刺激B细胞。为了明确B细胞功能障碍及其调节条件，对4例持续性全身性淋巴结病（PGL）患者和HIV阳性血清进行了高纯度B细胞的功能研究。数据与艾滋病患者及正常对照者的数据进行了比较。实验包括对一种非T细胞依赖性多克隆B细胞激活剂（PBA）[肺炎克雷伯菌，KlebsM]的分化反应（培养上清液中IgM/G的分泌）的体外研究。在刺激条件下IgM/G水平微弱升高是其特征。添加重组白细胞介素2（rIL2）未能提高自发免疫球蛋白水平。然而，使用KlebsM和rIL2的共培养实验导致免疫球蛋白水平与健康个体相似。明显患有艾滋病的患者IgG分泌未增加。这表明PGL患者以及部分艾滋病患者（仅IgM）中，rIL2可调节对PBA的异常B细胞分化反应。
