Porwit A, Panayotides P, Wasserman J, Reizenstein P
Department of Medicine, Karolinska Hospital, Stockholm, Sweden.
Cancer Detect Prev. 1988;12(1-6):91-6.
Peripheral blood mononuclear cells (PBMC) from patients with acute myeloid leukemia (AML) or non-Hodgkin lymphoma (NHL) and healthy controls were stimulated with phytohemagglutinin (PHA) and interleukin-2 (IL-2). PHA (1 microgram/ml) induced higher 3H-thymidine incorporation than 800 U/ml IL-2 in PBMC from both controls and patients with AML in complete remission. A synergistic effect between PHA and IL-2 was found. Malignant B cells from 5/12 NHL expressed IL-2 receptors and showed proliferative response to IL-2, but not to PHA. PHA induced higher cytotoxicity toward AML blasts in lymphocytes from healthy controls than did IL-2. In addition, PHA induced higher cytotoxicity in lymphocytes from healthy controls than in those from patients with AML in remission. In contrast, no difference in cytotoxicity between controls' and patients' lymphocytes was found after stimulation with IL-2. No HLA restriction could be demonstrated. Normal peripheral blood mononuclear cells and leukemic blasts from 2/12 AML were completely resistant to cytotoxic cells even at effector:target ratios four times as high as those otherwise required.
用植物血凝素（PHA）和白细胞介素-2（IL-2）刺激急性髓系白血病（AML）患者、非霍奇金淋巴瘤（NHL）患者以及健康对照者的外周血单个核细胞（PBMC）。在完全缓解的AML患者和对照者的PBMC中，PHA（1微克/毫升）诱导的3H-胸腺嘧啶核苷掺入量高于800单位/毫升的IL-2。发现PHA和IL-2之间存在协同效应。12例NHL患者中有5例的恶性B细胞表达IL-2受体，并对IL-2有增殖反应，但对PHA无反应。PHA对健康对照者淋巴细胞中AML原始细胞的细胞毒性高于IL-2。此外，PHA对健康对照者淋巴细胞的细胞毒性高于缓解期AML患者的淋巴细胞。相比之下，用IL-2刺激后，对照者和患者淋巴细胞的细胞毒性没有差异。未显示出HLA限制。12例AML患者中有2例的正常外周血单个核细胞和白血病原始细胞即使在效应细胞：靶细胞比例比其他所需比例高四倍的情况下，对细胞毒性细胞也完全耐药。
