Schröder Agnes, Stumpf Julia, Paddenberg Eva, Neubert Patrick, Schatz Valentin, Köstler Josef, Jantsch Jonathan, Deschner James, Proff Peter, Kirschneck Christian
Department of Orthodontics, University Hospital Regensburg, Regensburg, Germany.
Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany.
BMC Oral Health. 2021 Aug 18;21(1):405. doi: 10.1186/s12903-021-01761-3.
Many adult orthodontic patients suffer from periodontitis, which is caused by oral pathogens such as the gram-negative Aggregatibacter actinomycetemcomitans (Agac). Like orthodontic tooth movement, periodontitis is associated with inflammation and alveolar bone remodelling thereby affecting orthodontic treatment. Interactions of both processes, however, are not sufficiently explored, particularly with regard to oxidative stress.
After preincubation with Agac lysate for 24 h periodontal ligament fibroblasts (PDLF) were either stretched or compressed for further 48 h simulating orthodontic forces in vitro. We analysed the expression of genes and proteins involved in the formation of reactive oxygen species (NOX-4, ROS) and nitric oxide (NOS-2), inflammation (TNF, IL-6, PTGS-2) and bone remodelling (OPG, RANKL).
Agac lysate elevated the expression of NOX-4, NOS-2, inflammatory IL-6 and PTGS-2 and the bone-remodelling RANKL/OPG ratio during compressive, but not tensile mechanical strain. Agac lysate stimulated pressure-induced inflammatory signalling, whereas surprisingly ROS formation was reduced. Pressure-induced downregulation of OPG expression was inhibited by Agac lysate.
Agac lysate impact on the expression of genes and proteins involved in inflammation and bone remodelling as well as ROS formation, when PDLF were subjected to mechanical forces occurring during orthodontic tooth movement.
许多成年正畸患者患有牙周炎，其由革兰氏阴性伴放线聚集杆菌（Agac）等口腔病原体引起。与正畸牙齿移动一样，牙周炎与炎症和牙槽骨重塑相关，从而影响正畸治疗。然而，这两个过程之间的相互作用尚未得到充分研究，特别是在氧化应激方面。
牙周膜成纤维细胞（PDLF）与Agac裂解物预孵育24小时后，在体外模拟正畸力，进一步拉伸或压缩48小时。我们分析了参与活性氧（NOX-4、ROS）和一氧化氮（NOS-2）形成、炎症（TNF、IL-6、PTGS-2）和骨重塑（OPG、RANKL）的基因和蛋白质的表达。
在压缩而非拉伸机械应变过程中，Agac裂解物升高了NOX-4、NOS-2、炎性IL-6和PTGS-2的表达以及骨重塑RANKL/OPG比值。Agac裂解物刺激压力诱导的炎症信号传导，而令人惊讶的是ROS形成减少。Agac裂解物抑制了压力诱导的OPG表达下调。
当PDLF受到正畸牙齿移动过程中产生的机械力作用时，Agac裂解物会影响参与炎症、骨重塑以及ROS形成的基因和蛋白质的表达。
