Immunity and Inflammation Group, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New Caledonia, France.
Methods Mol Biol. 2020;2134:243-255. doi: 10.1007/978-1-0716-0459-5_22.
Experimental infections greatly contribute to further deepen our knowledge of infectious diseases. In the case of leptospirosis, hamsters as well as gerbils and guinea pigs have been used as animal models of acute leptospirosis in studying the pathophysiology of the disease. Here we describe a typical Leptospira infection using golden Syrian hamsters. We will also present techniques we use to study the resulting bacterial burden and gene expression patterns in the host in order to decipher the innate immune response to leptospirosis.
实验感染极大地有助于进一步加深我们对传染病的认识。在钩端螺旋体病的情况下，仓鼠以及沙鼠和豚鼠已被用作研究疾病病理生理学的急性钩端螺旋体病的动物模型。在这里，我们使用金黄地鼠描述了典型的钩端螺旋体感染。我们还将介绍我们用于研究宿主中细菌负荷和基因表达模式的技术，以破译对钩端螺旋体病的固有免疫反应。
