Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA, USA.
Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
Methods Mol Biol. 2020;2134:257-270. doi: 10.1007/978-1-0716-0459-5_23.
Leptospirosis is a major public health problem, especially in developing countries. Current vaccine studies focus on identifying Leptospira proteins that elicit protective immunity. Here, we describe a method to assess recombinant proteins for their ability to protect hamsters from fatal infection against Leptospira and to provide sterilizing immunity.
钩端螺旋体病是一个主要的公共卫生问题，特别是在发展中国家。目前的疫苗研究侧重于鉴定能够引发保护性免疫的钩端螺旋体蛋白。在这里，我们描述了一种评估重组蛋白的方法，以评估其保护仓鼠免受致命感染的能力，并提供杀菌免疫。
