Xiao Z-S, Long H, Zhao L, Li H-X, Zhang X-N
Department of Gastrointestinal Surgery, The First Affiliated Hospital of University of South China, Hengyang, China.
Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):6692-6698. doi: 10.26355/eurrev_202006_21656.
To explore the role of long-noncoding ribonucleic acid HOXA transcript at the distal tip (lncRNA HOTTIP) in the proliferation and apoptosis of gastric carcinoma cells.
The expressions of lncRNA HOTTIP in gastric carcinoma cell lines MGC-803, HGC-27, SNU-1, and SGC-7901 and normal gastric mucosa cell line RGM-1 were detected by real-time fluorescence quantitative polymerase chain reaction (PCR) and compared. The effects of lncRNA HOTTIP on proliferation and apoptosis of gastric carcinoma cells were detected by cell counting kit-8 (CCK-8), colony formation assay, and flow cytometry, respectively. StarBase v2.0 website was adopted to predict the relationship between lncRNA HOTTIP and target miRNAs. Dual-Luciferase reporter assay was performed to verify the sponge effect of lncRNA HOTTIP on miR-615-3p. CCK-8 experiment was conducted to detect its effect on proliferation of gastric carcinoma cells after co-silencing lncRNA HOTTIP and miR-615-3p.
LncRNA HOTTIP was highly expressed in gastric carcinoma cell lines MGC-803, HGC-27, SNU-1, and SGC-7901 than in normal gastric mucosa cell line RGM-1. After knockdown of lncRNA HOTTIP, the proliferation function of gastric carcinoma cells was markedly weakened, and the proportion of apoptotic cells increased. LncRNA HOTTIP was able to adsorb miR-615-3p via a sponge effect. Notably, knockdown of miR-615-3p restored the effect of silenced lncRNA HOTTIP on the proliferation function of gastric carcinoma cells.
LncRNA HOTTIP is highly expressed in gastric carcinoma cells. It affects cell proliferation and apoptosis in gastric carcinoma by adsorbing miR-615-3p via a sponge effect.
探讨长链非编码核糖核酸HOXA转录本远端末端（lncRNA HOTTIP）在胃癌细胞增殖和凋亡中的作用。
采用实时荧光定量聚合酶链反应（PCR）检测lncRNA HOTTIP在胃癌细胞系MGC-803、HGC-27、SNU-1和SGC-7901以及正常胃黏膜细胞系RGM-1中的表达，并进行比较。分别采用细胞计数试剂盒-8（CCK-8）、集落形成试验和流式细胞术检测lncRNA HOTTIP对胃癌细胞增殖和凋亡的影响。利用StarBase v2.0网站预测lncRNA HOTTIP与靶miRNA之间的关系。进行双荧光素酶报告基因试验以验证lncRNA HOTTIP对miR-615-3p的海绵效应。在lncRNA HOTTIP和miR-615-3p共沉默后，进行CCK-8实验检测其对胃癌细胞增殖的影响。
lncRNA HOTTIP在胃癌细胞系MGC-803、HGC-27、SNU-1和SGC-7901中的表达高于正常胃黏膜细胞系RGM-1。lncRNA HOTTIP敲低后，胃癌细胞的增殖功能明显减弱，凋亡细胞比例增加。lncRNA HOTTIP能够通过海绵效应吸附miR-615-3p。值得注意的是，miR-615-3p敲低恢复了沉默lncRNA HOTTIP对胃癌细胞增殖功能的影响。
lncRNA HOTTIP在胃癌细胞中高表达。它通过海绵效应吸附miR-615-3p影响胃癌细胞的增殖和凋亡。
