Mołoń Agnieszka, Podgórska Dominika, Płonka Artur, Bajorek Wojciech, Czarny Wojciech, Król Paweł, Podgórski Rafał, Cieśla Marek
Laboratory of Diagnostic and Clinical Epigenetics, Faculty of Medicine, University of Rzeszów, 2A Kopisto Ave., 35-959 Rzeszów, Poland.
Department of Rheumatology, Faculty of Medicine, University of Rzeszów, 16C Tadeusza Rejtana Ave., 35-959 Rzeszów, Poland.
Int J Mol Sci. 2025 Aug 21;26(16):8086. doi: 10.3390/ijms26168086.
Athletic performance is modulated by a complex interaction of physiological, environmental, and genetic factors, with regular exercise triggering molecular changes that influence gene expression and tissue adaptation. Despite growing knowledge, the underlying molecular mechanisms remain only partially understood, highlighting the need for precise biomarkers to monitor training-induced physiological adaptations. Long non-coding RNAs (lncRNAs) regulate cellular processes, including adaptation to physical exercise. Twelve healthy elite female volleyball players (mean age 27 ± 5.4 years) participated in the study. This study evaluated the expression of selected lncRNAs (SNHG4, SNHG5, PACERR, NEAT1, HIX003209, and HOTTIP) during a 10-week training program and evaluated their potential as biomarkers of training adaptation. Blood samples were collected before and after the training period. LncRNA expression was measured by quantitative polymerase chain reaction. HOTTIP exhibited an increase in expression after training (over sixfold change, = 0.009, adjusted = 0.024) and demonstrated high diagnostic accuracy (AUC = 0.917), which improved to 0.97 when combined with creatine kinase. Other lncRNAs showed no significant changes, although a correlation between HOTTIP and SNHG4 was noted. HOTTIP is markedly upregulated following chronic exercise and, especially when combined with creatine kinase, shows promise as a molecular biomarker for monitoring training adaptation in elite female volleyball players.
运动表现受生理、环境和遗传因素的复杂相互作用调节，规律运动引发影响基因表达和组织适应的分子变化。尽管知识不断增长，但潜在的分子机制仍仅部分为人所知，这凸显了需要精确的生物标志物来监测训练引起的生理适应。长链非编码RNA（lncRNA）调节细胞过程，包括对体育锻炼的适应。十二名健康的精英女排运动员（平均年龄27±5.4岁）参与了该研究。本研究评估了在为期10周的训练计划中选定lncRNA（SNHG4、SNHG5、PACERR、NEAT1、HIX003209和HOTTIP）的表达，并评估了它们作为训练适应生物标志物的潜力。在训练期前后采集血样。通过定量聚合酶链反应测量lncRNA表达。HOTTIP在训练后表达增加（变化超过六倍，P = 0.009，校正后P = 0.024），并显示出高诊断准确性（AUC = 0.917），与肌酸激酶联合时提高到0.97。其他lncRNA未显示出显著变化，尽管注意到HOTTIP与SNHG4之间存在相关性。长期运动后HOTTIP明显上调，尤其是与肌酸激酶联合时，有望作为监测精英女排运动员训练适应的分子生物标志物。
