Intensive Care Unit, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, PR. China.
Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):6980-6993. doi: 10.26355/eurrev_202006_21690.
In recent years, studies have shown that noncoding RNA (circRNA) is an important regulatory molecule involved in cell physiology and pathology. Herein, we analyzed the role of circRNA-68566 in the regulation of myocardial ischemia-reperfusion (I/R) injury by regulating miR-6322/PARP2 signaling pathway.
Cell viability was checked by CCK-8; LDH concentration, ROS production, MDA, SOD and GSH-Px were measured by corresponding kits; QPCR was used to inspect the expression of circRNA-0068566 and miR-6322 in I/R injury and H9C2 cells; luciferase reporter assay confirmed the direct target effect of circRNA-0068566 and miR-6322; Western blot was used to investigate PARP2 protein expression in I/R injury and H9C2 cells.
We analyzed the regulatory effect of circRNA-68566 on I/R injury and found that circRNA-68566 promoted the proliferation of injured cardiomyocytes in vitro and in vivo. circRNA-68566 and miR-6322 were directly combined to regulate the development of I/R injury. We also confirmed that PARP2 was the target of miR-6322 in I/R injury.
We believed that circRNA-68566 participated in myocardial ischemia-reperfusion injury by regulating miR-6322/PARP2 signaling pathway, which provided a new possible strategy for the treatment of I/R injury.
近年来的研究表明，非编码 RNA（circRNA）是参与细胞生理和病理的重要调节分子。在此，我们通过调控 miR-6322/PARP2 信号通路分析 circRNA-68566 在调节心肌缺血再灌注（I/R）损伤中的作用。
通过 CCK-8 检测细胞活力；通过相应试剂盒检测 LDH 浓度、ROS 产生、MDA、SOD 和 GSH-Px；通过 QPCR 检测 I/R 损伤和 H9C2 细胞中 circRNA-0068566 和 miR-6322 的表达；通过荧光素酶报告实验验证 circRNA-0068566 和 miR-6322 的直接靶标效应；通过 Western blot 检测 I/R 损伤和 H9C2 细胞中 PARP2 蛋白表达。
我们分析了 circRNA-68566 对 I/R 损伤的调节作用，发现 circRNA-68566 促进了体外和体内受损心肌细胞的增殖。circRNA-68566 和 miR-6322 直接结合，调节 I/R 损伤的发生。我们还证实，PARP2 是 miR-6322 在 I/R 损伤中的靶标。
我们认为 circRNA-68566 通过调控 miR-6322/PARP2 信号通路参与心肌缺血再灌注损伤，为 I/R 损伤的治疗提供了新的可能策略。
