Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Giza, Egypt.
Microbiology Department, Central Laboratory for Environmental Quality-Monitoring (CLEQM), National Water Research Center, Cairo, Egypt.
Prep Biochem Biotechnol. 2021;51(1):16-27. doi: 10.1080/10826068.2020.1786698. Epub 2020 Jul 7.
The spore laccase enzyme production by was optimized. It was characterized and tested for its textile dye decolorization potential. LB medium was found to be the most promising growth medium with addition of glucose (1-2%), yeast extract (0.1%), FeCl (0.01 mM) and MnCl (0.001 mM). The optimum spore laccase production was at pH 8, 30 °C, 1:5 medium to air ratio, 2% inoculum size and 7 days incubation. The characterization study of the enzyme showed the maximum activity at 60 °C and pH 6-7.5. It was induced by Ca, Mg, Fe, Zn, Cu and Na at 1 mM concentration. Also, it was stable in the presence of methanol, ethanol, acetone and chloroform. In addition, it enhanced about 34% by 5 mM HO and it was nearly stable at 10-20 mM HO. Furthermore, mediators such as ABTS, syrengaldazine and 2, 6 dimethyl phenol enhanced the spore laccase activity. The spore laccase enzyme efficiently decolorized direct red 81 and acid black 24 after 24 h. Phytotoxicity of the direct red 81 solution after decolorization by tested spore laccase was lower than that of the untreated dye solution. Finally, this study added a promising spore laccase candidate for ecofriendly and cost-effective dye wastewater bio-decolorization.
通过 优化了孢子漆酶的生产。对其进行了特性分析，并测试了其用于纺织染料脱色的潜力。发现 LB 培养基是最有前途的生长培养基，添加葡萄糖（1-2%）、酵母提取物（0.1%）、FeCl（0.01mM）和 MnCl（0.001mM）。孢子漆酶的最佳生产条件为 pH8、30°C、1:5 培养基与空气比、2%接种量和 7 天孵育。酶的特性研究表明，最大活性在 60°C 和 pH6-7.5。它在 1mM 浓度下被 Ca、Mg、Fe、Zn、Cu 和 Na 诱导。此外，它在甲醇、乙醇、丙酮和氯仿存在下稳定。此外，HO 增加了约 34%，HO 浓度在 10-20mM 时几乎稳定。此外，ABTS、愈创木酚紫和 2,6-二甲基苯酚等介体增强了孢子漆酶的活性。孢子漆酶酶能有效脱色直接红 81 和酸性黑 24，24 小时后即可完成。经测试的孢子漆酶脱色后，直接红 81 溶液的植物毒性低于未处理染料溶液。最后，这项研究为环保、经济有效的染料废水生物脱色增加了一种有前途的孢子漆酶候选物。
