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一种具有高热稳定性的漆酶，具有出色的孔雀石绿脱色解毒能力。

An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green.

机构信息

Department of Microbiology, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.

The Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450002, China.

出版信息

Int J Mol Sci. 2021 Oct 29;22(21):11755. doi: 10.3390/ijms222111755.

DOI:10.3390/ijms222111755
PMID:34769185
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8583942/
Abstract

Laccases can catalyze the remediation of hazardous synthetic dyes in an eco-friendly manner, and thermostable laccases are advantageous to treat high-temperature dyeing wastewater. A novel laccase from (Ghlac) was cloned and expressed in . Ghlac containing 263 residues was characterized as a functional laccase of the DUF152 family. By structural and biochemical analyses, the conserved residues H78, C119, and H136 were identified to bind with one copper atom to fulfill the laccase activity. In order to make it more suitable for industrial use, Ghlac variant Mut2 with enhanced thermostability was designed. The half-lives of Mut2 at 50 °C and 60 °C were 80.6 h and 9.8 h, respectively. Mut2 was stable at pH values ranging from 4.0 to 8.0 and showed a high tolerance for organic solvents such as ethanol, acetone, and dimethyl sulfoxide. In addition, Mut2 decolorized approximately 100% of 100 mg/L of malachite green dye in 3 h at 70 °C. Furthermore, Mut2 eliminated the toxicity of malachite green to bacteria and . In summary, the thermostable laccase Ghlac Mut2 could effectively decolorize and detoxify malachite green at high temperatures, showing great potential to remediate the dyeing wastewater.

摘要

漆酶可以以环保的方式催化修复危险的合成染料，而耐热漆酶有利于处理高温染色废水。从 （Ghlac）克隆并表达了一种新型漆酶 。Ghlac 含有 263 个残基，被鉴定为具有 DUF152 家族功能的漆酶。通过结构和生化分析，确定了保守残基 H78、C119 和 H136 与一个铜原子结合，以实现漆酶活性。为了使其更适合工业用途，设计了具有增强耐热性的 Ghlac 变体 Mut2。Mut2 在 50°C 和 60°C 下的半衰期分别为 80.6 h 和 9.8 h。Mut2 在 pH 值为 4.0 至 8.0 的范围内稳定，并对乙醇、丙酮和二甲基亚砜等有机溶剂具有高耐受性。此外，Mut2 在 70°C 下 3 小时内可将 100mg/L 的孔雀石绿染料脱色约 100%。此外，Mut2 消除了孔雀石绿对细菌和 的毒性。总之，耐热漆酶 Ghlac Mut2 可以有效地在高温下对孔雀石绿进行脱色和解毒，在修复染色废水方面具有很大的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a8f/8583942/4775b6f2f252/ijms-22-11755-g007.jpg
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Int J Biol Macromol. 2021 Nov 1;190:574-584. doi: 10.1016/j.ijbiomac.2021.09.003. Epub 2021 Sep 8.
3
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4
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Indian J Microbiol. 2021 Mar;61(1):45-54. doi: 10.1007/s12088-020-00912-4. Epub 2020 Nov 19.
7
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