Kimata H, Sherr E H, Saxon A
Department of Medicine, UCLA School of Medicine 90024.
J Clin Immunol. 1988 Sep;8(5):381-9. doi: 10.1007/BF00917154.
The supernatant of unstimulated purified NKH-1 bearing human natural killer (NK) cells was found to enhance ongoing immunoglobulin synthesis. This NK-Cell supernatant (NKSN) enhanced IgE, IgG, and IgA synthesis from corresponding B-cell lines without increasing thymidine incorporation or cell number. Separation of NKH-1+ cells into CD3- or CD3+ cells showed that this activity was produced by the CD3- population. Recombinant human interleukin (IL)-1, IL-2, IL-4, interferon (INF)-beta 1, INF-gamma, granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor (TNF)-alpha, or partially purified low molecular weight B-cell growth factor (BCGF) failed to provide the same enhancement of Ig synthesis. While the NKSN contained small amounts of IL-6 (0.1 U/ml) and IL-6 could increase Ig synthesis in vitro, the optimal IL-6 enhancement was far less than that observed with NKSN. NKSN also enhanced ongoing Ig synthesis from in vivo activated B cells obtained from peripheral blood or bone marrow but failed to induce Ig synthesis from resting or in vitro activated B cells. These results demonstrate that human NK (CD3-, NKH-1+) cells can produce B-cell differentiation activity capable of regulating Ig production in vivo, which appears to be distinct from the activity of previously described cytokines.
未受刺激的携带人自然杀伤（NK）细胞的纯化NKH-1细胞的上清液被发现可增强正在进行的免疫球蛋白合成。这种NK细胞上清液（NKSN）可增强相应B细胞系中IgE、IgG和IgA的合成，而不会增加胸苷掺入或细胞数量。将NKH-1+细胞分离为CD3-或CD3+细胞表明，这种活性是由CD3-群体产生的。重组人白细胞介素（IL）-1、IL-2、IL-4、干扰素（INF）-β1、INF-γ、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、肿瘤坏死因子（TNF）-α或部分纯化的低分子量B细胞生长因子（BCGF）均未能提供相同的Ig合成增强作用。虽然NKSN含有少量IL-6（0.1 U/ml），且IL-6可在体外增加Ig合成，但最佳的IL-6增强作用远低于NKSN所观察到的。NKSN还增强了从外周血或骨髓获得的体内活化B细胞正在进行的Ig合成，但未能诱导静止或体外活化B细胞的Ig合成。这些结果表明，人NK（CD3-，NKH-1+）细胞可产生能够在体内调节Ig产生的B细胞分化活性，这似乎与先前描述的细胞因子的活性不同。
