Sharon M, Gnarra J R, Baniyash M, Leonard W J
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.
J Immunol. 1988 Nov 15;141(10):3512-5.
In the process of evaluating murine hybridomas for an antibody to the beta-subunit of the IL-2R (p70) we identified an antibody that immunoprecipitated a 55- to 57-kDa complex from cross-linked lysates. We demonstrate that this complex is composed of IL-2 (15.5 kDa) cross-linked to the H chain of HLA class I (40 to 42 kDa), suggesting a molecular interaction between HLA class I molecules and IL-2R. Although the exact role of this association remains to be determined, the specific cross-linking of IL-2 to HLA class I Ag is intriguing in view of published claims for a role of HLA class I in OKT3-induced lymphocyte proliferation and in NK cell lytic activity.
在评估鼠杂交瘤产生的针对白细胞介素-2受体（p70）β亚基的抗体的过程中，我们鉴定出一种抗体，该抗体能从交联裂解物中免疫沉淀出一个55至57 kDa的复合物。我们证明，该复合物由与HLA I类重链（40至42 kDa）交联的白细胞介素-2（15.5 kDa）组成，提示HLA I类分子与白细胞介素-2受体之间存在分子相互作用。尽管这种关联的确切作用尚待确定，但鉴于已发表的关于HLA I类在OKT3诱导的淋巴细胞增殖和NK细胞裂解活性中作用的说法，白细胞介素-2与HLA I类抗原的特异性交联很有趣。
