Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Bioresour Technol. 2020 Oct;314:123760. doi: 10.1016/j.biortech.2020.123760. Epub 2020 Jul 1.
To reveal nitrogen removal mechanisms under environmental stresses, biofilm reactors were operated at different temperatures (10 °C-35 °C) treating saline wastewater (salinity 3%). The results showed nitrogen removal efficiency was 98.46% at 30 °C and 60.85% at 10 °C, respectively. Both ammonia oxidizing archaea (AOA) and ammonia oxidizing bacteria (AOB) participated in nitrification. 94.9% of the overall ammonia oxidation was attributed to AOA at 10 °C, but only 48.2% of that was undertaken by AOA at 35 °C. AOA had a greater contribution at low temperature, which demonstrated that nitrogen removal pathway varied with temperature. Aerobic denitrification was more stable than anoxic denitrification. High-throughput sequencing showed Crenarchaeota was the dominant AOA (97.02-34.47%), cooperating with various heterotrophic AOB. Real-time PCR indicated that AOA was three orders of magnitude more abundant than AOB. AOA was more resistant to low temperature and high-saline stresses. Ammonia oxidizers had distinct responses to temperature change and showed diverse relationships at different temperatures.
为了揭示环境胁迫下的氮去除机制，采用生物膜反应器在不同温度（10°C-35°C）下处理含盐废水（盐度 3%）。结果表明，30°C 时氮去除效率为 98.46%，10°C 时为 60.85%。氨氧化古菌（AOA）和氨氧化细菌（AOB）均参与硝化作用。10°C 时，总氨氧化的 94.9%归因于 AOA，但 35°C 时仅 48.2%归因于 AOA。AOA 在低温下的贡献更大，这表明氮去除途径随温度而变化。好氧反硝化比缺氧反硝化更稳定。高通量测序表明，Crenarchaeota 是优势 AOA（97.02-34.47%），与各种异养 AOB 合作。实时 PCR 表明，AOA 的丰度比 AOB 高三个数量级。AOA 对低温和高盐胁迫具有更强的抗性。氨氧化菌对温度变化有明显的响应，并在不同温度下表现出不同的关系。
