Department of Basic and Medical Sciences, Neurosciences and Sense Organs, Section of Human Anatomy and Histology, University of Bari, 70124 Bari, Italy.
Department of Biomedical Science and Human Oncology, Paediatric Unit, University of Bari, 70100 Bari, Italy.
Int J Mol Sci. 2020 Jul 3;21(13):4739. doi: 10.3390/ijms21134739.
Obesity may affect bone health, but literature reports are contradictory about the correlation of body mass index (BMI) and bone markers. LIGHT, one of the immunostimulatory cytokines regulating the homeostasis of bone and adipose tissue, could be involved in obesity. The study involved 111 obese subjects (12.21 ± 3.71 years) and 45 controls. Patients underwent the evaluation of bone status by quantitative ultrasonography (QUS). LIGHT amounts were evaluated in sera by ELISA, whereas its expression on peripheral blood cells was evaluated by flow cytometry. Osteoclastogenesis was performed by culturing peripheral blood mononuclear cells (PBMCs) with or without anti-LIGHT antibodies. Obese patients showed significant high BMI-standard deviation score (SDS), weight-SDS, and Homeostatic model assessment for insulin resistance (HOMA-IR) that negatively correlated with the reduced Amplitude Dependent Speed of Sound (AD-SoS)-Z-score and Bone Transmission Time (BTT-Z)-score. They displayed significantly higher serum levels of LIGHT compared with controls (497.30 ± 363.45 pg/mL vs. 186.06 ± 101.41 pg/mL, < 0.001). LIGHT expression on monocytes, CD3+-T-cells, and neutrophils was also higher in obese patients than in the controls. Finally, in PBMC cultures, the addition of anti-LIGHT antibodies induced a significant osteoclastogenesis inhibition. Our study highlighted the high serum levels of LIGHT in obese children and adolescents, and its relationship with both the grade of obesity and bone impairment.
肥胖可能会影响骨骼健康，但文献报道关于体重指数（BMI）与骨标志物的相关性存在矛盾。调节骨和脂肪组织平衡的免疫刺激细胞因子 LIGHT 可能与肥胖有关。该研究纳入了 111 名肥胖受试者（12.21±3.71 岁）和 45 名对照者。患者通过定量超声（QUS）评估骨骼状况。通过 ELISA 评估血清中的 LIGHT 含量，通过流式细胞术评估其在外周血单个核细胞上的表达。通过培养外周血单个核细胞（PBMCs）并用或不用抗 LIGHT 抗体来进行破骨细胞生成。肥胖患者的 BMI-标准差评分（SDS）、体重-SDS 和胰岛素抵抗稳态模型评估（HOMA-IR）显著较高，与降低的声速衰减依赖性速度（AD-SoS）-Z 评分和骨传输时间（BTT-Z）-Z 评分呈负相关。与对照组相比，肥胖患者的血清 LIGHT 水平显著较高（497.30±363.45 pg/mL 比 186.06±101.41 pg/mL， <0.001）。与对照组相比，肥胖患者的单核细胞、CD3+T 细胞和中性粒细胞上的 LIGHT 表达也更高。最后，在 PBMC 培养中，加入抗 LIGHT 抗体可显著抑制破骨细胞生成。我们的研究强调了肥胖儿童和青少年血清中高水平的 LIGHT，及其与肥胖程度和骨损伤的关系。
