Cox N R, Davidson M K, Davis J K, Lindsey J R, Cassell G H
Department of Comparative Medicine, University of Alabama, Birmingham 35294.
Lab Anim Sci. 1988 Aug;38(4):381-8.
For 4 years a colony of cesarean-derived, isolator-maintained LEW/Tru rats was evaluated for mycoplasmal infection by serology, culture and histopathology. Anti-mycoplasmal antibodies were detected by enzyme-linked immunosorbent assay (ELISA), and the colony eventually was found to have inapparent infections of Mycoplasma pulmonis and Mycoplasma arthritidis. Rats, naturally infected with M. pulmonis, remained consistently positive in the M. pulmonis ELISA after their initial seroconversion, and eventually developed clinical signs and lesions of respiratory and genital mycoplasmosis. M. pulmonis was apparently eliminated by serological testing and removal of infected rats. Rats naturally infected with M. arthritidis did not develop clinical or histologic evidence of mycoplasmal disease and their sera gave inconsistent results in the M. pulmonis ELISA, but eventually developed positive M. arthritidis ELISA responses. M. arthritidis was isolated from the genital tract, the intestinal tract, and Harderian gland. In contrast to M. pulmonis, removal of serologically positive animals was not sufficient for elimination of M. arthritidis from the colony.
在4年的时间里，通过血清学、培养和组织病理学方法，对一群剖宫产获得、饲养于隔离器中的LEW/Tru大鼠进行了支原体感染评估。采用酶联免疫吸附测定（ELISA）检测抗支原体抗体，最终发现该大鼠群体存在隐性的肺支原体和关节炎支原体感染。自然感染肺支原体的大鼠在初次血清转化后，肺支原体ELISA检测始终呈阳性，最终出现了呼吸道和生殖系统支原体病的临床症状和病变。通过血清学检测和剔除感染大鼠，肺支原体明显被清除。自然感染关节炎支原体的大鼠未出现支原体病的临床或组织学证据，其血清在肺支原体ELISA检测中结果不一致，但最终关节炎支原体ELISA检测呈阳性反应。从生殖道、肠道和哈德氏腺中分离出了关节炎支原体。与肺支原体不同，剔除血清学阳性动物不足以从群体中清除关节炎支原体。
