Huang Jie, Liu Yan, Mao Ke, Gu Qing, Wu Xingwei
Department of Ophthalmology, Shanghai General Hospital of Nanjing Medical University, Shanghai, China.
Department of Ophthalmology, Baoshan Hospital of Integrated Traditional Chinese Medicine and Western Medicine, Shanghai, China.
Mol Vis. 2020 Jun 27;26:494-504. eCollection 2020.
To observe the effects of tetramethylpyrazine (TMP) on mice retinas injured by sodium iodate (NaIO).
Male mice (n = 45) were randomly divided into three groups: the control group (Group C), the NaIO-degenerated group (Group I), and the TMP-treated group (TMP group). The Group I mice were intraperitoneally injected with 35 mg/kg NaIO. The Group C mice were injected with similar volumes of PBS. The TMP group mice were intraperitoneally injected with 80 mg/kg TMP starting 24 h after NaIO administration once a day for 14 days. Fundus photography, optical coherence tomography (OCT), electroretinography (ERG), hematoxylin and eosin (H&E) staining, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, and western blotting were used to assess the effects of TMP on mice retinas at day 3, 7, and 14 after NaIO administration.
TMP effectively prevented the decrease in the thicknesses of the retinas and the outer nuclear layer (ONL), and effectively alleviated the functional decline in the retinas after NaIO administration. TMP significantly decreased the number of TUNEL-positive cells in retinas. In addition, TMP rapidly increased the expression of Nrf2 and HO-1 and decreased BAX expression in mice retinas after NaIO injection.
TMP alleviates morphological and functional retinal damage in mice exposed to NaIO and reduces retinal apoptosis.
观察川芎嗪（TMP）对碘酸钠（NaIO）损伤小鼠视网膜的影响。
将45只雄性小鼠随机分为三组：对照组（C组）、NaIO退变组（I组）和TMP治疗组（TMP组）。I组小鼠腹腔注射35 mg/kg NaIO。C组小鼠注射等量体积的PBS。TMP组小鼠在NaIO给药后24小时开始腹腔注射80 mg/kg TMP，每天一次，共14天。在NaIO给药后第3、7和14天，采用眼底照相、光学相干断层扫描（OCT）、视网膜电图（ERG）、苏木精-伊红（H&E）染色、末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）检测和蛋白质免疫印迹法评估TMP对小鼠视网膜的影响。
TMP有效预防了NaIO给药后视网膜和外核层（ONL）厚度的降低，并有效减轻了视网膜的功能衰退。TMP显著减少了视网膜中TUNEL阳性细胞的数量。此外，TMP快速增加了NaIO注射后小鼠视网膜中Nrf2和HO-1的表达，并降低了BAX的表达。
TMP减轻了暴露于NaIO的小鼠视网膜的形态和功能损伤，并减少了视网膜细胞凋亡。
