基于基因去重复化驱动发现深绿木霉中三萜烯醇酸生物合成途径。
Genetic dereplication driven discovery of a tricinoloniol acid biosynthetic pathway in Trichoderma hypoxylon.
作者信息
Liu Huan, Pu Yu-Han, Ren Jin-Wei, Li Er-Wei, Guo Li-Xia, Yin Wen-Bing
机构信息
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, PR China.
Environmental and Resources Institute, Chongqing Technology and Business University, Chongqing 400067, PR China.
出版信息
Org Biomol Chem. 2020 Jul 22;18(28):5344-5348. doi: 10.1039/d0ob01202e.
A genetic dereplication approach in combination with differential gene expression led to the discovery of three new sesquiterpenes, tricinoloniol acids (TRAs) A-C (1-3) and the known fusidilactone A (4) from T. hypoxylon. Comparative transcriptomic analysis and targeted deletion identified the biosynthetic route for TRAs. Our results demonstrate an alternative application of the genetic dereplication method for exploring the biosynthesis of cryptic secondary metabolites (SMs), which utilizes the coordinated expression of trichothecene (tri) and tra cluster genes.
一种基因去重复方法与差异基因表达相结合，从低柄曲霉中发现了三种新的倍半萜类化合物，即曲古菌素酸（TRAs）A - C（1 - 3）以及已知的夫西地内酯A（4）。比较转录组分析和靶向缺失确定了TRAs的生物合成途径。我们的结果证明了基因去重复方法在探索隐秘次生代谢产物（SMs）生物合成中的另一种应用，该方法利用了单端孢霉烯族毒素（tri）和tra簇基因的协同表达。
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