Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-yama, Sendai 980-8578, Japan.
Org Lett. 2012 Jan 20;14(2):513-5. doi: 10.1021/ol203097b. Epub 2011 Dec 27.
The concomitant addition of the histone deacetylase inhibitor and the DNA methyltransferase inhibitor to the culture medium of an entomopathogenic fungus, Isaria tenuipes, greatly enhanced its secondary metabolite production and led to the isolation of tenuipyrone (1), a novel polyketide with an unprecedented tetracyclic ring system bearing a spiroketal structural component, along with two known C(10)-polyketides, cephalosporolide B (2), which is a plausible biosynthetic precursor of 1, and cephalosporolide F (3).
同时向昆虫病原真菌——薄束梗孢（Isaria tenuipes）的培养基中添加组蛋白去乙酰化酶抑制剂和 DNA 甲基转移酶抑制剂，极大地促进了其次生代谢产物的产生，并从中分离到了一种新型的多酮化合物—— tenuipyrone（1），它具有一个前所未有的四环系统，带有螺缩酮结构成分，以及另外两种已知的 C(10)-多酮化合物——头孢洛利德 B（2）和头孢洛利德 F（3）。头孢洛利德 B 是 1 的一个可能的生物合成前体。
