Zuppardo Marcelo Lelis, Santamaria Milton, Ferreira Camila Lopes, Longo Mariéllen, Cirelli Joni Augusto, Santamaria Mauro Pedrine, Jardini Maria Aparecida Neves
Universidade Estadual Paulista, São José dos Campos, São Paulo, Brasil.
Programa de Pós-Graduação em Ortodontia, Centro Universitário Hermínio Ometto, UNIARARAS, Araras, Brasil.
J Appl Oral Sci. 2020;28:e20190766. doi: 10.1590/1678-7757-2019-0766. Epub 2020 Jul 3.
Objective To compare two corticotomy surgical protocols in rats to verify whether they alter conventional orthodontic movement. Methodology Sixty Wistar rats were divided into three groups - orthodontic movement (CG), orthodontic movement and corticotomy (G1) and orthodontic movement with corticotomy and decortication (G2) - and euthanized after 7 and 14 days. Tooth movement (mm), bone volume fraction and bone volume ratio to total volume (BV/TV), and bone mineral density (BMD) were evaluated by micro-CT. The total amount of bone was measured in square millimeters and expressed as the percentage of bone area in the histomorphometry. The number of positive TRAP cells and RANK/RANKL/OPG interaction were also investigated. Results Day 14 showed a statistically significant difference in orthodontic tooth movement in CG compared with G1 (7.52 mm; p=0.009) and G2 (7.36 mm; p=0.016). A micro-CT analysis revealed a difference between CG, G1 and G2 regarding BV/TV, with G1 and G2 presenting a lower BV/TV ratio at 14 days (0.77 and 0.73 respectively); we found no statistically significant differences regarding BMD. There was a difference in the total amount of bone in the CG group between 7 and 14 days. At 14 days, CG presented a significantly higher bone percentage than G1 and G2. Regarding TRAP, G2 had more positive cells at 7 and 14 days compared with CG and G1. Conclusion Corticotomy accelerates orthodontic movement. Decortication does not improve corticotomy efficiency.
目的 比较大鼠的两种皮质切开术手术方案，以验证它们是否会改变传统的正畸移动。方法 将60只Wistar大鼠分为三组——正畸移动组（CG）、正畸移动加皮质切开术组（G1）和正畸移动加皮质切开术及去皮质术组（G2），并在7天和14天后实施安乐死。通过显微CT评估牙齿移动（毫米）、骨体积分数以及骨体积与总体积之比（BV/TV）和骨矿物质密度（BMD）。以平方毫米为单位测量骨总量，并在组织形态计量学中表示为骨面积的百分比。还研究了抗酒石酸酸性磷酸酶阳性细胞数量以及RANK/RANKL/OPG相互作用。结果 第14天显示，CG组与G1组（7.52毫米；p = 0.009）和G2组（7.36毫米；p = 0.016）相比，正畸牙齿移动存在统计学显著差异。显微CT分析显示，CG组、G1组和G2组在BV/TV方面存在差异，G1组和G2组在第14天的BV/TV比值较低（分别为0.77和0.73）；我们发现BMD方面无统计学显著差异。CG组在7天至14天之间骨总量存在差异。在第14天，CG组的骨百分比明显高于G1组和G2组。关于抗酒石酸酸性磷酸酶，与CG组和G1组相比，G2组在第7天和第14天有更多阳性细胞。结论 皮质切开术可加速正畸移动。去皮质术并不能提高皮质切开术的效率。
