College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
PLoS One. 2020 Jul 8;15(7):e0214497. doi: 10.1371/journal.pone.0214497. eCollection 2020.
The Bashbay sheep (Ovis aries), an indigenous breed of Xinjiang, China, has many excellent characteristics. It is resistant to Mycoplasma ovipneumoniae infection, the causative agent of mycoplasma ovipneumonia, a chronic respiratory disease that is harmful to the sheep industry. To date, knowledge regarding the mechanisms responsible for M. ovipneumoniae pathogenesis in scant. Herein, we report the results of transcriptome profiling of lung tissues from Bashbay sheep experimentally infected with an M. ovipneumoniae strain at 4 and 14 days post-infection, in comparison to mock-infected animals (0 d). Transcriptome profiling was performed by deep RNA sequencing, using the Illumina platform. The analysis of differentially expressed genes was performed to determine concomitant gene-specific temporal patterns of mRNA expression in the lungs after M. ovipneumoniae infection. We found 1048 differentially expressed genes (575 up-regulated, 473 down-regulated) when comparing transcriptomic data at 4 and 0 days post-infection, and 2823 (1362 up-regulated, 1461 down-regulated) when comparing 14 versus 0 days post-infection. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the differentially expressed genes at 4 and 14 versus 0 days post-infection were enriched in 245 and 287 pathways, respectively, and the Toll-like receptor (TLR) signaling pathway was considered most closely related to MO infection (p < 0.01). Two pathways (LAMP-TLR2/TLR6-MyD88-MKK6-AP1-IL1B and LAMP-TLR8MyD88-IRF5-RANTES) were identified based on the TLR signaling pathway from differentially expressed genes related M. ovipneumoniae infection. Gene Ontology analysis showed that differentially expressed genes in different groups were enriched for 1580 and 4561 terms, where those most closely related to M. ovipneumoniae infection are positive regulators of inflammatory responses (p < 0.01). These results could aid in understanding how M. ovipneumoniae infection progresses in the lungs and may provide useful information regarding key regulatory pathways.
新疆巴尔楚克羊（Ovis aries）是一种地方品种，具有许多优良特性。它对绵羊肺炎支原体（Mycoplasma ovipneumoniae）感染具有抗性，绵羊肺炎支原体是一种慢性呼吸道疾病，对绵羊养殖业有害。迄今为止，关于绵羊肺炎支原体发病机制的知识还很少。在此，我们报告了在感染绵羊肺炎支原体后 4 天和 14 天与模拟感染（0 天）的巴尔楚克羊的肺组织进行转录组分析的结果。通过使用 Illumina 平台进行深度 RNA 测序进行转录组分析。对差异表达基因的分析用于确定绵羊肺炎支原体感染后肺部特定基因的 mRNA 表达的伴随时间模式。在比较感染后 4 天和 0 天的转录组数据时，我们发现了 1048 个差异表达基因（575 个上调，473 个下调），而在比较 14 天和 0 天的转录组数据时，发现了 2823 个差异表达基因（1362 个上调，1461 个下调）。京都基因与基因组百科全书途径分析表明，在感染后 4 天和 14 天与 0 天相比，差异表达基因分别富集在 245 和 287 个途径中，而 Toll 样受体（TLR）信号途径被认为与 MO 感染最为密切相关（p < 0.01）。基于与绵羊肺炎支原体感染相关的差异表达基因，从 TLR 信号途径中鉴定出两条途径（LAMP-TLR2/TLR6-MyD88-MKK6-AP1-IL1B 和 LAMP-TLR8MyD88-IRF5-RANTES）。基因本体论分析表明，不同组中的差异表达基因分别富集了 1580 和 4561 个术语，其中与绵羊肺炎支原体感染最密切相关的是炎症反应的正调节剂（p < 0.01）。这些结果有助于了解绵羊肺炎支原体感染在肺部的进展情况，并可能为关键调控途径提供有用的信息。
