Laboratory of Micro- and Nanostructured Systems, Institute of Chemistry of New Materials of National Academy of Sciences of Belarus, Minsk, Belarus.
Cell Molecular Biology Laboratory, Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus, Minsk, Belarus.
Biomed Mater. 2020 Nov 21;15(6):065022. doi: 10.1088/1748-605X/aba40a.
In the present study, ultrathin poly(L-lactic acid) (PLLA) films were fabricated using the spin-coating technique. Physicochemical properties of the formed materials, including their morphology, thickness, transparency, and contact angle, have been studied. We determined that the morphology of PLLA films could be regulated by changing the polymer concentration and humidity. By altering the humidity, microporous and flat PLLA films can be fabricated. The obtained samples were subsequently used for culturing mesenchymal stem cells and fibroblasts. It has been determined that cells effectively adhered to prepared films and formed on them a monolayer culture with high viability. It has been shown that PLLA films are suitable for the entrapment of curcumin (up to 12.1 μm cm) and provide its sustained release in solutions isotonic to blood plasma. The obtained PLLA films appear to be prospective materials for potential application in regenerative medicine as part of cell-containing tissue engineered dressings for chronic wound treatment.
在本研究中，使用旋涂技术制备了超薄聚（L-乳酸）（PLLA）薄膜。研究了形成材料的物理化学性质，包括其形态、厚度、透明度和接触角。我们发现，通过改变聚合物浓度和湿度可以调节 PLLA 薄膜的形态。通过改变湿度，可以制备出微孔和平整的 PLLA 薄膜。随后，将获得的样品用于培养间充质干细胞和成纤维细胞。结果表明，细胞能够有效地附着在制备的薄膜上，并在其上形成具有高活力的单层培养物。结果表明，PLLA 薄膜适合姜黄素（高达 12.1 μm cm）的包封，并在与血浆等渗的溶液中提供其持续释放。所得的 PLLA 薄膜似乎是再生医学中作为包含细胞的组织工程敷料的一部分用于慢性伤口治疗的有前途的材料。
