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L.的α-淀粉酶抑制、抗氧化、抗菌潜力及植物化学成分评估

Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of L.

作者信息

Nasir Abdul, Khan Mushtaq, Rehman Zainab, Khalil Atif Ali Khan, Farman Saira, Begum Naeema, Irfan Muhammad, Sajjad Wasim, Parveen Zahida

机构信息

Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.

Department of Molecular Science and Technology, Ajou University, Suwan 16499, Korea.

出版信息

Plants (Basel). 2020 Jul 6;9(7):852. doi: 10.3390/plants9070852.

Abstract

L. is a traditionally used medicinal plant. The present study was designed to explore the α-amylase inhibitory, antioxidant, and antimicrobial activities of L. Polarity-based solvent extracts (-hexane, acetone, chloroform, methanol, ethanol, and water) of leaves and stem were used. Antioxidant activity was assessed by free radical scavenging assay (FRAP) and 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity methods. Quantitative phytochemical analyses suggested that the stem of L. contains higher levels of bioactive compounds than its leaves ( < 0.05). The results suggested that stem-derived extracts of L. are more active against bacterial species, including two Gram-positive and three Gram-negative strains. Moreover, our results showed that the bioactive compounds of L. significantly inhibit α-amylase activity. Finally, we reported the polarity-based solvent extracts of L. and revealed that the stem, rather than leaves, has a high antioxidant potential as measured by FRAP and DPPH assay with IC values of 1.38 and 1.59 mg/mL, respectively. It may also be deducted from the data that the L. could be a significant candidate, which should be subjected to further isolation and characterization, to be used as an antidiabetic, antimicrobial and antioxidant resource in many industries, like food, pharmaceuticals and cosmetics.

摘要

L. 是一种传统上使用的药用植物。本研究旨在探索L. 的α -淀粉酶抑制、抗氧化和抗菌活性。使用了基于极性的溶剂提取物(正己烷、丙酮、氯仿、甲醇、乙醇和水)对其叶和茎进行提取。通过自由基清除测定法(FRAP)和2,2 -二苯基苦味酰基自由基清除活性方法评估抗氧化活性。定量植物化学分析表明,L. 的茎中生物活性化合物的含量高于其叶(P < 0.05)。结果表明,L. 茎提取物对包括两种革兰氏阳性菌和三种革兰氏阴性菌在内的细菌种类更具活性。此外,我们的结果表明,L. 的生物活性化合物能显著抑制α -淀粉酶活性。最后,我们报道了L. 的基于极性的溶剂提取物,并揭示通过FRAP和DPPH测定法,茎而非叶具有较高的抗氧化潜力,IC值分别为1.38和1.59 mg/mL。从数据中还可以推断,L. 可能是一个重要的候选物,应进一步进行分离和表征,以便在食品、制药和化妆品等许多行业中用作抗糖尿病、抗菌和抗氧化资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9871/7412011/73068658bbc4/plants-09-00852-g001.jpg

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