Faculty of Pharmacy, Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Nat Prod Res. 2022 Jan;36(2):610-616. doi: 10.1080/14786419.2020.1788555. Epub 2020 Jul 8.
In this work, -hexane, chloroform, and ethyl acetate fractions of the methanol extract of Houtt. seeds were evaluated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) Ellman's method. It was found that all fractions depicted no anti-AChE activity, however, they were active toward BChE with IC values of 361.8, 215.0, and 145.8 µg/mL, respectively comparing with donepezil as the reference drug (IC = 1.97 µg/mL). The ethyl acetate fraction which also showed high neuroprotectivity and metal chelating ability was selected for the phytochemical analysis. Our results confirmed the presence of trimyristin and 5,7-diacetyl chrysin (reported for the first time in ) in the corresponding fraction.
在这项工作中，我们评估了甲醇提取物的 -己烷、氯仿和乙酸乙酯馏分对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的抑制活性，采用 Ellman 的方法进行测定。结果表明，所有馏分均未表现出抗 AChE 活性，但对 BChE 具有活性，IC 值分别为 361.8、215.0 和 145.8μg/mL，与作为参比药物的多奈哌齐（IC = 1.97μg/mL）相比。由于乙酸乙酯馏分还显示出较高的神经保护活性和金属螯合能力，因此选择其进行植物化学分析。我们的结果证实了在相应馏分中存在三肉豆蔻酸甘油酯和 5,7-二乙酰基白杨素（首次在 中报道）。
