Manayi Azadeh, Rastegari Arezoo, Heydarian Bita, Vahedi-Mazdabadi Yasaman, Khanavi Mahnaz, Akbarzadeh Tahmineh, Saeedi Mina
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Nat Prod Res. 2023 Apr;37(7):1227-1231. doi: 10.1080/14786419.2021.1999944. Epub 2021 Nov 2.
The methanol extract of L. was fractionated using -hexane, chloroform, and ethyl acetate to evaluate their cholinesterase (ChE) inhibitory activity modified Ellman's method. It was perceived that only the ethyl acetate fraction was active toward acetylcholinesterase (AChE) with IC value of 172.21 µg/mL. Also, all fractions showed no butyrylcholinesterase (BChE) inhibitory activity. The ethyl acetate fraction was also investigated for its neuroprotectivity and metal chelating ability (Zn, Fe, and Cu) which demonstrated desired activity. Phytochemical analysis of the ethyl acetate fraction led to isolation and identification of formononetin 7--β-D-glucopyranoside which has not been previously reported for this plant.
使用正己烷、氯仿和乙酸乙酯对L.的甲醇提取物进行分级分离，以采用改良的Ellman方法评估其胆碱酯酶（ChE）抑制活性。结果发现，只有乙酸乙酯级分对乙酰胆碱酯酶（AChE）具有活性，IC值为172.21 µg/mL。此外，所有级分均未显示出对丁酰胆碱酯酶（BChE）的抑制活性。还对乙酸乙酯级分的神经保护作用和金属螯合能力（锌、铁和铜）进行了研究，结果表明其具有预期的活性。对乙酸乙酯级分进行植物化学分析，分离并鉴定出了刺芒柄花素7-O-β-D-吡喃葡萄糖苷，该化合物此前尚未在该植物中报道过。
