乌墨和多香果对紫色色杆菌群体感应的抑制作用
Inhibition of quorum sensing in Chromobacterium violaceum by Syzygium cumini L. and Pimenta dioica L.
作者信息
Vasavi Halkare Suryanarayana, Arun Ananthapadmanabha Bhagwath, Rekha Punchapady Devasya
机构信息
Yenepoya Research Center, Yenepoya University, University Road, Nityananda Nagar, Deralakatte, Mangalore-575018, India.
出版信息
Asian Pac J Trop Biomed. 2013 Dec;3(12):954-9. doi: 10.1016/S2221-1691(13)60185-9.
OBJECTIVE
To investigated into the anti-quorum sensing (QS) activity of Syzygium cumini L. (S. cumini) and Pimenta dioica L. (P. dioica) using Chromobacterium violaceum (C. violaceum) strains.
METHODS
In this study, anti-QS activity of ethanol extract of Syzygium cumini L. and Pimenta dioica L. were screened using C. violaceum CV026 biosensor bioassay. By bioassay guided fractionation of S. cumini and P. dioica, ethyl acetate fraction (EAF) with strong anti-QS activity was separated. Inhibition of QS regulated violacein production in C. violaceum ATCC12472 by EAF was assessed at different concentrations. The effect of EAF on the synthesis of autoinducer like N-acyl homoserine lactone (AHL) was studied in C. violaceum ATCC31532 using its mutant C. violaceum CV026 by standard methods.
RESULTS
EAF inhibited violacein production in C. violaceum ATCC12472 in a concentration dependent manner without significant reduction in bacterial growth. Complete inhibition of violacein production was evidenced in 0.75-1.0 mg/mL concentration of EAF without inhibiting the synthesis of the AHL. TLC biosensor overlay profile of EAF revealed two translucent spots in S. cumini and P. dioica that inhibited C6-AHL mediated violacein production in C. violaceum CV026.
CONCLUSIONS
This study indicates the anti-QS activity of the tested medicinal plants against C. violaceum.
目的
利用紫色杆菌菌株研究乌墨（Syzygium cumini L.）和多香果（Pimenta dioica L.）的群体感应（QS）抑制活性。
方法
在本研究中，使用紫色杆菌CV026生物传感器生物测定法筛选乌墨和多香果乙醇提取物的QS抑制活性。通过生物测定指导的乌墨和多香果分级分离，分离出具有强QS抑制活性的乙酸乙酯级分（EAF）。评估不同浓度的EAF对紫色杆菌ATCC12472中QS调控的紫菌素产生的抑制作用。采用标准方法，在紫色杆菌ATCC31532及其突变体紫色杆菌CV026中研究EAF对类自诱导物N-酰基高丝氨酸内酯（AHL）合成的影响。
结果
EAF以浓度依赖的方式抑制紫色杆菌ATCC12472中紫菌素的产生，而细菌生长没有显著降低。在0.75 - 1.0 mg/mL浓度的EAF中可证明紫菌素产生被完全抑制，且不抑制AHL的合成。EAF的薄层色谱生物传感器覆盖图谱显示，乌墨和多香果中有两个半透明斑点，可抑制紫色杆菌CV026中C6 - AHL介导的紫菌素产生。
结论
本研究表明受试药用植物对紫色杆菌具有QS抑制活性。
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