Cruise J L, Michalopoulos G
J Cell Physiol. 1985 Oct;125(1):45-50. doi: 10.1002/jcp.1041250107.
Primary cultures of adult rat hepatocytes are stimulated to enter DNA synthesis by norepinephrine (NE). This stimulation is maximal if the hepatocytes are incubated with NE for more than 12 hr, beginning no later than 2-4 hr after the cells are first plated. After 24 hr in culture, hepatocytes are unresponsive to NE stimulation. A strong synerergistic interaction between NE and epidermal growth factor (EGF) may be observed in cultures incubated with both EGF and NE, or pretreated with NE, then exposed to EGF. This interaction may be related to the finding that NE, in similarity with other factors that enhance EGF stimulation, reduces binding at the EGF receptor during the first 24 hr in culture.
去甲肾上腺素（NE）可刺激成年大鼠肝细胞的原代培养物进入DNA合成。如果肝细胞在首次接种后不迟于2 - 4小时开始与NE孵育超过12小时，这种刺激作用最大。培养24小时后，肝细胞对NE刺激无反应。在同时用表皮生长因子（EGF）和NE孵育的培养物中，或先用NE预处理，然后再暴露于EGF的培养物中，可观察到NE与表皮生长因子（EGF）之间有强烈的协同相互作用。这种相互作用可能与以下发现有关：与其他增强EGF刺激的因子类似，NE在培养的最初24小时内会减少EGF受体处的结合。
