Illian Didi Nurhadi, Hasibuan Poppy Anjelisa Zaitun, Sumardi Sumardi, Nuryawan Arif, Wati Ridha, Basyuni Mohammad
Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
Department of Apothecary, Faculty of Pharmacy, University of Tjut Nyak Dhien, Medan, Indonesia.
Iran J Pharm Res. 2019 Summer;18(3):1477-1487. doi: 10.22037/ijpr.2019.1100719.
Colorectal cancer is the third most common cancer world wide and has been occurred more in developing regions. The use of conventional chemotherapy agents may lead to various adverse effects. Therefore, it is required to find the potential drug for anticancer from alternative source of natural product including mangrove plants. The present study was conducted to determine the anticancer activity of polyisoprenoids from Blume. leaves (PAL) in WiDr cells. Cell cycle inhibition, apoptosis activity, and suppression of cyclooxygenase-2 (COX-2) were also evaluated. The anticancer activity of PAL was determined by observing the activity of these compounds against WiDr cells using the [3-(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazolium bromida] MTT assay. Inhibition of the cell cycle and increased apoptosis were analysed by flowcytometry. Suppression of COX-2 was analysed using immunocytochemistry. PAL exhibited anticancer activity against WiDr cells with an IC of 173.775 μg/mL. Cell cycle analysis revealed that the inhibition occurred in the G0-G1 phase, and apoptosis occurred in the early apoptosis phase. Furthermore, the result of an analysis of COX-2 expression showed that PAL enabled the suppression of COX-2 expression. PAL can be used as anticancer agents against WiDr colon cancer cells. However, studies is required to confirm the finding of the anticancer activity of polyisoprenoid extract.
结直肠癌是全球第三大常见癌症，在发展中地区更为多发。使用传统化疗药物可能会导致各种不良反应。因此，需要从包括红树林植物在内的天然产物替代来源中寻找潜在的抗癌药物。本研究旨在确定来自布鲁姆叶（PAL）的聚异戊二烯类化合物在WiDr细胞中的抗癌活性。还评估了细胞周期抑制、凋亡活性和环氧合酶-2（COX-2）的抑制情况。通过使用[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑]MTT法观察这些化合物对WiDr细胞的活性来确定PAL的抗癌活性。通过流式细胞术分析细胞周期抑制和凋亡增加情况。使用免疫细胞化学分析COX-2的抑制情况。PAL对WiDr细胞表现出抗癌活性，IC值为173.775μg/mL。细胞周期分析显示抑制发生在G0-G1期，凋亡发生在早期凋亡阶段。此外，COX-2表达分析结果表明PAL能够抑制COX-2表达。PAL可作为抗WiDr结肠癌细胞的抗癌剂。然而，需要进一步研究来证实聚异戊二烯提取物抗癌活性的这一发现。
