Aboukazempour Amiri Mohsen, Aghamaali Mahmoud Reza, Parsian Hadi, Tashakkorian Hamed
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
Department of Basic Sciences, Mazandaran University of Science & Technology (MUST), Babol, Iran.
Iran J Pharm Res. 2020 Fall;19(4):394-409. doi: 10.22037/ijpr.2020.112680.13890.
Many attempts in medical community focused on the preparation of anticancer agents. Various Coenzyme Q such as CoQ analogs have been reported as anti-inflammatory, anticancer, and antioxidant substances. In this study a novel derivatives of Coenzyme Q as an anticancer agent have been introduced. The prepared magnetic nanoparticle, containing CoQ were prepared using common chemical methods and also characterized by means of nuclear magnetic resonance (NMR), fourier transform infrared (FT-IR), thermal gravimetric analysis (TGA), and differential scanning calorimetric (DSC). To evaluate the antiproliferative effects of the nanoparticle, the prepared compound was treated with cell lines such as Hela, MCF-7 and Saos. Moreover, the outcomes were compared with normal fibroblast cell line. These assessments were performed by means of MTT assay. Investigation on the capability of this prepared nanoparticle showed some reliable results including cytotoxicities against MCF7, Saos and Hela cancer cell lines which were illustrated by displaying the morphology of the treated cells using AO/EB dual staining fluorescent technique. Employing simple method for preparation as well as the promising cytotoxic results makes it as a promising candidate for further bioexperiments.
医学界进行了许多尝试，专注于抗癌药物的制备。各种辅酶Q，如辅酶Q类似物，已被报道具有抗炎、抗癌和抗氧化作用。在本研究中，引入了一种新型的辅酶Q衍生物作为抗癌剂。使用常见化学方法制备了含辅酶Q的磁性纳米颗粒，并通过核磁共振（NMR）、傅里叶变换红外光谱（FT-IR）、热重分析（TGA）和差示扫描量热法（DSC）对其进行了表征。为了评估纳米颗粒的抗增殖作用，将制备的化合物用HeLa、MCF-7和Saos等细胞系进行处理。此外，将结果与正常成纤维细胞系进行比较。这些评估通过MTT法进行。对该制备的纳米颗粒性能的研究显示了一些可靠的结果，包括对MCF7、Saos和HeLa癌细胞系的细胞毒性，通过使用AO/EB双染荧光技术展示处理后细胞的形态得以说明。采用简单的制备方法以及有前景的细胞毒性结果使其成为进一步生物实验的有希望的候选物。
