Westermann J, Peschel P, Pabst R
Center of Anatomy, Medical School of Hannover, Federal Republic of Germany.
Cell Tissue Res. 1988 Nov;254(2):403-13. doi: 10.1007/BF00225813.
Inbred rats were used as a model to determine the influence of the age of the implanted splenic tissue and the age of the host on the structure of transplanted splenic tissue. Monoclonal antibodies against lymphocyte, macrophage and dendritic cell subsets were used to evaluate the different compartments of the spleen. Adult rats received implants from adult, weanling or fetal rats, weanling rats received splenic tissue from adult, weanling or fetal rats and neonatal rats received neonatal or fetal spleens. There were major differences in the structure and cellular composition of the regenerated splenic tissue. The younger the recipients and the donor spleens, the better the normalization of the splenic compartments and the less fibrous tissue was found 3 months after transplantation. The follicles regenerated in all transplants, but the marginal zone was only normally developed in wealing and neonatal hosts. The periarteriolar lymphatic sheath regenerated in a similar manner to the marginal zone. Whenever a compartment developed, its cellular composition was the same as in a normal spleen. The immunohistological techniques enabled splenic regeneration to be characterized revealing a far from normal histological splenic structure in many age groups. These findings suggest that splenic regeneration in children might result in splenic tissue with normal compartments, which would be in contrast to some data in adults.
近交系大鼠被用作模型，以确定植入脾组织的年龄和宿主年龄对移植脾组织结构的影响。使用针对淋巴细胞、巨噬细胞和树突状细胞亚群的单克隆抗体来评估脾脏的不同区域。成年大鼠接受来自成年、断奶或胎鼠的植入物，断奶大鼠接受来自成年、断奶或胎鼠的脾组织，新生大鼠接受新生或胎脾。再生脾组织的结构和细胞组成存在重大差异。受体和供体脾脏越年轻，脾区的正常化越好，移植后3个月发现的纤维组织越少。所有移植中都有卵泡再生，但边缘区仅在断奶和新生宿主中正常发育。动脉周围淋巴鞘的再生方式与边缘区相似。每当一个区域发育时，其细胞组成与正常脾脏相同。免疫组织学技术能够对脾再生进行表征，揭示许多年龄组的脾组织结构远非正常。这些发现表明，儿童的脾再生可能会产生具有正常区域的脾组织，这与成人的一些数据形成对比。
