Bergmann E S, Leitner W, Brtko J, Boeckl O, Thalhamer J
Walter Reed Army Institute of Research, Department of Immunology, Washington, USA.
Physiol Res. 1997;46(1):1-8.
The present paper deals with the regeneration of splenic tissue after autologous transplantation. Control and transplanted rats (60 days after operation (10(6) cells per injection). The effect of a primary response was studied by a single injection, long-lasting bacteraemia was imitated by 5 injections in weekly intervals. Spleens and transplants were investigated by flow-cytometry and immunohistochemistry. Additionally, the proliferation activity and the specific antibody production against Escherichia coli proteins were tested. Flow-cytometric analysis showed altered behaviour of T-helper cells and B-cells in transplants following a primary response, whereas in the multiple injection group a difference between the splenic and transplant response was restricted to macrophages and MHC II+ cells. The results of the morphometric analysis revealed that the cellular composition of unstimulated transplants was very similar to that of the spleen with some subtle alterations. Only the marginal zone showed more striking differences concerning the homing of several cell classes. Under stimulatory conditions, these subtle alterations became more drastic so that CD5+ cells, B-cells and macrophages responded in an abnormal manner in both groups. The analysis of thymidine kinase disclosed decreased activity in the spleen after weekly antigen stimulation. The stimulation index of all transplant groups was significantly lower than that of the spleen. The specific antibody (IgG) production after a single immunization was highest in the transplant group. All groups responded after the multiple challenge. In conclusion, the results demonstrate that splenic transplants differs in several, but subtle aspects from normal splenic tissue. The main reason for most of these alterations may be a slightly misguided recirculation and/or homing of cells.
本文探讨自体移植后脾组织的再生情况。对照组和移植组大鼠（术后60天，每次注射10(6)个细胞）。通过单次注射研究初次反应的效果，通过每周注射5次模拟持续性菌血症。采用流式细胞术和免疫组织化学方法研究脾脏和移植组织。此外，检测了增殖活性以及针对大肠杆菌蛋白的特异性抗体产生情况。流式细胞术分析显示，初次反应后移植组织中T辅助细胞和B细胞的行为发生改变，而在多次注射组中，脾脏和移植组织反应的差异仅限于巨噬细胞和MHC II+细胞。形态计量分析结果显示，未受刺激的移植组织的细胞组成与脾脏非常相似，只是存在一些细微变化。仅边缘区在几种细胞类型的归巢方面存在更明显差异。在刺激条件下，这些细微变化变得更加显著，以至于两组中的CD5+细胞、B细胞和巨噬细胞均出现异常反应。胸苷激酶分析显示，每周抗原刺激后脾脏中的活性降低。所有移植组的刺激指数均显著低于脾脏。单次免疫后移植组中特异性抗体（IgG）的产生最高。多次攻击后所有组均有反应。总之，结果表明脾移植在几个方面与正常脾组织存在差异，但较为细微。这些改变的主要原因可能是细胞的再循环和/或归巢略有偏差。
