State Key Laboratory of Urban Water Resource and Environment, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China; Micro- and Nanotechnology Research Center, Harbin Institute of Technology, Harbin 150080, China.
Micro- and Nanotechnology Research Center, Harbin Institute of Technology, Harbin 150080, China.
Anal Chim Acta. 2016 Apr 15;916:77-83. doi: 10.1016/j.aca.2016.02.019. Epub 2016 Feb 23.
We present a facile fabrication of layer-by-layer (LbL) microarrays of quantum dots (QDs) and acetylcholinesterase enzyme (AChE). The resulting arrays had several unique properties, such as low cost, high integration and excellent flexibility and time-saving. The presence of organophosphorous pesticides (OPs) can inhibit the AChE activity and thus changes the fluorescent intensity of QDs/AChE microscopic dot arrays. Therefore, the QDs/AChE microscopic dot arrays were used for the sensitive visual detection of OPs. Linear calibration for parathion and paraoxon was obtained in the range of 5-100 μg L(-1) under the optimized conditions with the limit of detection (LOD) of 10 μg L(-1). The arrays have been successfully used for detection of OPs in fruits and water real samples. The new array was validated by comparison with conventional high performance liquid chromatography-mass spectrometry (HPLC-MS).
我们提出了一种简便的量子点（QD）和乙酰胆碱酯酶（AChE）层层（LbL）微阵列的制备方法。所得的阵列具有几个独特的性质，例如低成本、高集成度以及极好的灵活性和省时性。有机磷农药（OPs）的存在会抑制 AChE 的活性，从而改变 QD/AChE 微观点阵列的荧光强度。因此，QD/AChE 微观点阵列被用于 OPs 的灵敏可视化检测。在优化条件下，对 parathion 和 paraoxon 的线性校准范围为 5-100μg/L，检测限（LOD）为 10μg/L。该阵列已成功用于水果和水实际样品中 OPs 的检测。通过与传统高效液相色谱-质谱（HPLC-MS）的比较，验证了新的阵列。
